STOCK Tg(Igk*A)A02Dgs/J

Stock number: 008720
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

A tandem series of adenine/thymine (A/T) repeats were introduced to an Igk (immunoglobulin Κ Vk167) transgene. This "TgA" line has an A/T sequence comparable to that found in line "TgG" without the single cytosine:guanine (C:G) insert (see Stock No. 008719) and does not show any increase in number of mutations in the activated B cell A/T repeat region of 4-12 month old mice. This strain may be useful in studying somatic hypermutations associated with the rapid diversification of antibodies during immune responses to antigen.

Detailed description

Alternating EcoRV and PvuII sites and a tandem series of adenine/thymine (A/T) repeats with a single guanine:cytosine (G:C) base pair were introduced to an Igk (immunoglobulin kappa; VΚ167) transgene. The cytosine (C) residue marks a site at which deamination to uracil (U) can occur, initiating somatic hypermutations of upstream and downstrean A/T residues. This "TgA" line has an A/T sequence comparable to that found in line "TgG" without the single cytosine:guanine (C:G) insert (see Stock No. 008719 ) and does not show any increase in number of mutations in the activated B cell A/T repeat region of 4-12 month old mice. This strain may be useful in studying somatic hypermutations associated with the rapid diversification of antibodies during immune responses to antigen. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

Development

VΚ167/PEPS, indirectly derived from BALB/c myeloma protein MPOC 167, was designed to carry alternating EcoRV and PvuII sites (EPS) in a transgene with a truncated region upstream of the promoter.

The Igk (immunoglobulin kappa) sequence was further modified in the current strain through the insertion of 5 tandem repeats of a 20-bp adenine/thymine (A/T) fragment. This "TgA" line has an A/T sequence comparable to that found in line "TgG" without the single cytosine:guanine (C:G) insert (see Stock No. 008719).

The transgene was initially introduced to a C57BL/6-SJL background, and subsequently crossed to BALB/cByJ or C57BL/6 inbred mice for a total of 8 generations by the donating investigators.

Allele

Symbol: Tg(Igk*A)A02Dgs
Name: transgene insertion A02, David G Schatz
MGI accession ID: MGI:3811481
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Igk*A)A02Dgs
Marker name: transgene insertion A02, David Schatz
Chromosome: UN
Strain of origin: C57BL/6 and SJL
Expressed genes: Igk,immunoglobulin kappa chain complex,mouse, laboratory
Molecular note: Vk167/PEPS is a transgene with a truncated region upstream of the promoter. The Igk (immunoglobulin kappa) sequence was further modified in the current strain through the insertion of 5 tandem repeats of a 20-bp adenine/thymine (A/T) fragment near the 3' end of the variable region. This "TgA" line has an A/T sequence comparable to that found in line "TgG" without the single cytosine:guanine (C:G) insert.