STOCK Tg(Igk*G)G07Dgs/J

Stock number: 008719
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

A tandem series of adenine/thymine (A/T) repeats with a single guanine:cytosine (G:C) base pair were introduced to an Igk (immunoglobulin kappa; Vk167) transgene. The cytosine (C) residue marks a site at which deamination to uracil (U) can occur, initiating somatic hypermutations of upstream and downstrean A/T residues. This "TgG" line, in which the cytosine was on the 3'->5' strand of the gene, showed very few mutations in the activated B cell A/T repeat region of 4-12 month old mice. This strain may be useful in studying somatic hypermutations associated with the rapid diversification of antibodies during immune responses to antigen.

Detailed description

Alternating EcoRV and PvuII sites and a tandem series of adenine/thymine (A/T) repeats with a single guanine:cytosine (G:C) base pair were introduced to an Igk (immunoglobulin kappa; VΚ167) transgene. The cytosine (C) residue marks a site at which deamination to uracil (U) can occur, initiating somatic hypermutations of upstream and downstrean A/T residues. This "TgG" line, in which the cytosine was on the 3'->5' strand of the gene, showed very few mutations in the activated B cell A/T repeat region of 4-12 month old mice. This strain may be useful in studying somatic hypermutations associated with the rapid diversification of antibodies during immune responses to antigen. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

Development

VΚ167/PEPS, indirectly derived from BALB/c myeloma protein MPOC 167, was designed to carry alternating EcoRV and PvuII sites (EPS) in a transgene with a truncated region upstream of the promoter.

The Igk (immunoglobulin kappa) sequence was further modified in the current strain through the insertion of 5 tandem repeats of a 20-bp adenine/thymine (A/T) fragment including a single guanine:cytosine (G:C) base pair in the central repeat. In this "TgG" line, the cytosine was placed on the 3'->5' strand of the DNA.

The transgene was initially introduced to a C57BL/6-SJL background, and subsequently crossed to BALB/cByJ or C57BL/6 inbred mice for a total of 8 generations by the donating investigators.

Allele

Symbol: Tg(Igk*G)G07Dgs
Name: transgene insertion G07, David G Schatz
MGI accession ID: MGI:3811466
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: TgG
Marker symbol: Tg(Igk*G)G07Dgs
Marker name: transgene insertion G07, David Schatz
Marker synonyms: TgG
Chromosome: UN
Strain of origin: C57BL/6 and SJL
Expressed genes: Igk,immunoglobulin kappa chain complex,mouse, laboratory
Molecular note: Vk167/PEPS is an Igk transgene with a truncated region upstream of the promoter. The Igk (immunoglobulin kappa) sequence was further modified through the insertion, near the 3' end of the variable region of Igk, of 5 tandem repeats of a 20-bp adenine/thymine (A/T) fragment in the same orientation as the insert in the TgA line but includes a single guanine:cytosine (G:C) base pair in the central repeat. In this "TgG" line, the cytosine was placed on the 3'->5' strand of the DNA.