Mice carrying this targeted mutation of Ywhae (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide) exhibit hippocampal defects, neuronal migration abnormalities, and increased apoptosis in the brain. This mutant mouse strain may be useful in studies of Miller-Dieker Lissencephaly Syndrome and neuronal migration during brain development.
Dr. Anthony Wynshaw-Boris, Case Western Reserve University, School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ywhae | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide |
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Most homozygotes die at birth, less than 1% of homozygotes survive to adulthood. Survivors remain smaller in size than wildtype controls. No gene product (protein) is detected by immunoblot analysis of brain tissue from homozygotes. Homozygotes exhibit hippocampal defects with thinning of the cortex, hippocampal pyramidal cell layer disorganization and neuronal migration abnormalities. Heterozygotes exhibit less severe hippocampal defects. Mutants have increased apoptosis in the brain. This mutant mouse strain may be useful in studies of Miller-Dieker Lissencephaly Syndrome and neuronal migration during brain development.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt parts of exons 2 and 3. The construct was electroporated into 129S6/SvEvTac derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to 129S6 mice. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ mice (Stock No. 002448) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Anthony Wynshaw-Boris |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | 14-3-3epsilon-; Ywhae- |
Gene Symbol and Name | Ywhae, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 11 |
Molecular Note | Exons 2 and 3 were partially replaced by a neomycin resistance gene and a loxP site. Immunoblot analysis indicated the complete absence of gene product in homozygotes and a reduction by half in heterozygotes. |
When maintaining a live colony, these mice can be bred as heterozygotes. Most homozygotes die at birth, less than 1% of homozygotes survive to adulthood.
When using the 129S-Ywhaetm1Awb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008715 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for whae<tm1Awb> |
Frozen Mouse Embryo | 129S-Ywhae<tm1Awb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129S-Ywhae<tm1Awb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129S-Ywhae<tm1Awb>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | 129S-Ywhae<tm1Awb>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.