These mutant mice harbor a knockout of the Epstein-Barr virus induced gene 3 (Ebi3) and may be useful in studying Foxp3+ regulatory T cell (Treg) function, IL12 heterodimeric cytokines (such as IL-35 and IL-27), IFNgamma production, and Th1/Th2 immune responses (including inflammatory bowel disease).
Richard S Blumberg, Brigham and Women's Hospital
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ebi3 | Epstein-Barr virus induced gene 3 |
Mice homozygous for this Ebi3-mutant allele are viable and fertile with the donating investigator reporting no overt autoimmunity or inflammatory disease associated with the mutation. No RNA from the targeted gene is observed in splenocytes isolated from homozygotes. Ebi3-deficiency leads to impaired Treg activity with failure to control homeostatic proliferation. Homozygotes exhibit decreased numbers and function of invariant natural killer T cells (iNKT); stimulated iNKT cells show impaired IL-4 production both in vivo and in vitro. Homozygotes are resistant to oxazolone-induced colitis (mediated primarily by T helper (Th) 2-type cytokine production by iNKT cells), whereas Ebi3-deficiency shows no affect on trinitrobenzene sulfonic acid-induced colitis (a predominantly Th1-mediated colitis model). These Ebi3-mutant strains may be useful in studying Treg function, IL12 heterodimeric cytokines (such as IL-35 and IL-27),IFNgamma production, and Th1/Th2 immune responses (including inflammatory bowel disease).
Of note, Ebi3-mutant mice are also be available on a BALB/c congenic background (see Stock No. 008701).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The targeting vector was designed to replace exons 2-5 (encoding amino acids 24-228) of the targeted gene with a PGK-Neo cassette. The donating investigators lab reports that the construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and offspring from the resulting chimeric mice were bred to C57BL/6NCrl inbred mice. Ebi3-mutant mice were then backcrossed to C57BL/6NCrl for at least 9 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the current colony.
Allele Name | targeted mutation 1, Richard Blumberg |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | EBI-3; EBI3-; EBI3tm Birk; Ebi3tm1Birk |
Gene Symbol and Name | Ebi3, Epstein-Barr virus induced gene 3 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 17 |
Molecular Note | Sequence encompassing exons 2 through 5 was replaced by a neomycin selection cassette inserted by homologous recombination. Northern blot analysis of splenic RNA indicated an absence of normal transcript in homozygous mutant mice. |
Mutations Made By | Richard Blumberg, Brigham and Women's Hospital |
When maintaining a live colony, homozygous mice may be bred together.
When using the EBI3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #008691 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous forEbi3<tm1Rsb> |
Frozen Mouse Embryo | B6.129X1-Ebi3<tm1Rsb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Ebi3<tm1Rsb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Ebi3<tm1Rsb>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Ebi3<tm1Rsb>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.