These mice express a MHC class II-restricted T-cell receptor recognizing the endogenous melanocyte differentiation antigen minimal TRP-1 (tyrosinase-related protein 1) epitope. This strain is a source for melanocyte reactive CD4+ cells from antigen-negative animals and may be useful in studies of cancer immunology and therapeutics.
Nicholas P. Restifo, National Cancer Institute
Genetic Background | Generation |
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N9+N2F12
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Radiation induced | Tyrp1 | tyrosinase-related protein 1 |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Rag1 | recombination activating gene 1 |
Allele Type |
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Transgenic (Inserted expressed sequence) |
Starting at:
$278.00 Domestic price for female 4-week |
556.00 Domestic price for breeder pair |
These mice are hemizygous for the TRP-1 transgene Tg(Tcra,Tcrb)9Rest, homozygous for the targeted mutation Rag1tm1Mom and homozygous for the white based brown radiation induced mutation of tyrosinase-related protein 1, Tyrp1B-w. These mutant mice express a MHC class II-restricted TCR recognizing the endogenous melanocyte differentiation antigen minimal TRP-1 (tyrosinase-related protein 1)
epitope corresponding to amino acids 113 to 127. The transgene is located on the Y chromosome in founder line 9. On a RAG-deficient background, these mice are also homozygous for the Tyrp1B-w mutation and do not produce endogenous tyrosinase-related protein 1. This strain is a source for melanocyte reactive CD4+ cells from antigen-negative animals and may be useful in studies of cancer immunology and therapeutics.
The TRP-1 TCR was generated from immunized Tyrp1B-w mice. Clone 7A6 expressed Valpha3.2Vbeta14 (Vα3.2 and Vβ14) TCR chains. These TCR sequences were cloned into separate vectors and co-injected into microinjected into C57BL/6 fertilized eggs. Founder line 9 was subsequently established and the transgene insertion site was found on the Y chromosome.
The Tyrp1B-w, white based brown mutation was generated in (101/RI x CJH/RI)FI exposed to ionizing radiation and involves the inversion at the 5' end of the tyrosinase-related protein 1 gene. The mice carrying the Tyrp1B-w mutation were backcrossed to the C57BL/6N background for 8 generations.
To generate this triple mutant strain, founder line 9 animals were crossed to mice with the Rag1tm1Mom targeted mutation on the C57BL/6 background. The mice were then crossed to mice carrying both the Rag1tm1Mom and Tyrp1B-w mutations. The strain is maintained hemizygous for the TRP-1 transgene Tg(Tcra,Tcrb)9Rest, homozygous for the targeted mutation Rag1tm1Mom and homozygous for the white based brown radiation induced mutation of tyrosinase-related protein 1, Tyrp1B-w.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 12, was segregating. Also, 2 of the 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6N;C57BL/6J genetic background.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
---|---|
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression |
Allele Name | white based brown |
---|---|
Allele Type | Radiation induced |
Allele Synonym(s) | b18FrS |
Gene Symbol and Name | Tyrp1, tyrosinase-related protein 1 |
Gene Synonym(s) | |
Strain of Origin | (101/Rl x C3H/Rl)F1 |
Chromosome | 4 |
Molecular Note | This allele has a gross DNA inversion at the 5' end of the gene. No detectable transcript is produced from this allele. |
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Rag-; Rag1tm1Mom; RAG-1-; Rag1-; RAG1null; Rag-1KO |
Gene Symbol and Name | Rag1, recombination activating gene 1 |
Gene Synonym(s) | |
Site of Expression | expression is seen in bone marrow derived cell lines. |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 2 |
Molecular Note | A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
Allele Name | transgene insertion 9, Nicholas P Restifo |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | TRP-1 TCR |
Gene Symbol and Name | Tg(Tcra,Tcrb)9Rest, transgene insertion 9, Nicholas P Restifo |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Strain of Origin | C57BL/6 |
Chromosome | Y |
Molecular Note | The TRP-1 TCR was generated from immunized Tyrp1B-w mice. Clone 7A6 expressed Valpha3.2Vbeta14 TCR chains. These TCR sequences were cloned into separate vectors and co-injected into microinjected into C57BL/6 fertilized eggs. Founder line 9 was subsequently established and the transgene insertion site was found on the Y chromosome. |
When maintained as a live colony, these mice strain can be bred as hemizygous for the TRP-1 transgene Tg(Tcra,Tcrb)9Rest, homozygous for the targeted mutation Rag1tm1Mom and homozygous for the white based brown radiation induced mutation of tyrosinase-related protein 1, Tyrp1B-w.
When using the RAG1- BW TRP-1 TCR mouse strain in a publication, please cite the originating article(s) and include JAX stock #008684 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Y linked Transgene.Homozygous for Rag1<tm1Mom>, Heterozygous for Tyrp1<B-w>, Females Non carrier and Males carriers for Tg(Tcra,Tcrb)9Rest |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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