B6.129S7-Atoh1^tm3Hzo/J

Stock number: 008681
Product name: Math1 flox
Research areas: Cell Biology Research, Neurobiology Research, Research Tools

Description

These mice possess loxP sites on either side of the coding region of the atonal homolog 1 gene (Atoh1). When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene and elucidating its role in cell fate commitment.

Detailed description

These mice possess loxP sites on either side of the coding region of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in further elucidating the role of this gene in cell fate commitment.

Development

A loxP site was inserted 300 bp upstream of the translocation start site, and a second was placed immediately 3' of the coding sequence, followed by an FRT-flanked PGK-neomycin selection cassette. The mutation was introduced to AB2.2 129S7/SvEvBrd-Hprt1⁺-derived embryonic stem (ES) cell line. Mice were crossed with an 129SvEv background hACT-Flpe strain to excise the selection cassette. This strain was backcrossed to C57BL/6 for more than ten generations by the donating laboratory.

Allele

Symbol: Atoh1^tm3Hzo
Name: targeted mutation 3, Huda Y Zoghbi
MGI accession ID: MGI:3767172
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Atoh1
Marker name: atonal bHLH transcription factor 1
Chromosome: 6
Strain of origin: 129S7/SvEvBrd-Hprt^b-m2
Molecular note: A loxP site was inserted 300 bp upstream of the translation start site and a second loxP site was inserted along with an Frt flanked neo cassette immediately 3' of the coding region. The neo cassette was removed by in vivo Flp-mediated recombination leaving the entire coding region flanked with loxP sites.