These mice possess loxP sites on either side of the coding region of the atonal homolog 1 gene (Atoh1). When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene and elucidating its role in cell fate commitment.
Huda Zoghbi, Baylor College of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Atoh1 | atonal bHLH transcription factor 1 |
These mice possess loxP sites on either side of the coding region of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in further elucidating the role of this gene in cell fate commitment.
A loxP site was inserted 300 bp upstream of the translocation start site, and a second was placed immediately 3' of the coding sequence, followed by an FRT-flanked PGK-neomycin selection cassette. The mutation was introduced to AB2.2 129S7/SvEvBrd-Hprt1+-derived embryonic stem (ES) cell line. Mice were crossed with an 129SvEv background hACT-Flpe strain to excise the selection cassette. This strain was backcrossed to C57BL/6 for more than ten generations by the donating laboratory.
Allele Name | targeted mutation 3, Huda Y Zoghbi |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Atoh1flox; Math1flox |
Gene Symbol and Name | Atoh1, atonal bHLH transcription factor 1 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 6 |
Molecular Note | A loxP site was inserted 300 bp upstream of the translation start site and a second loxP site was inserted along with an Frt flanked neo cassette immediately 3' of the coding region. The neo cassette was removed by in vivo Flp-mediated recombination leaving the entire coding region flanked with loxP sites. |
Mutations Made By | Huda Zoghbi, Baylor College of Medicine |
When maintained as a live colony, homozygotes may be bred.
When using the Math1 flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #008681 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Atoh1<tm3Hzo> |
Frozen Mouse Embryo | B6.129S7-Atoh1<tm3Hzo>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Atoh1<tm3Hzo>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Atoh1<tm3Hzo>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S7-Atoh1<tm3Hzo>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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