These Blm, Bloom syndrome homolog (human), mutant mice possess loxP sites on either side of exon 8 and may be useful in generating conditional mutations for studying Bloom Syndrome.
Dr. Philip Leder, Harvard Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Blm | Bloom syndrome, RecQ like helicase |
These mice possess loxP sites on either side of exon 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 8 deleted in the cre-expressing tissue(s).
When bred to a strain with Cre recombinase expression in mammary cells, or a strain with widespread Cre recombinase expression, this mutant mouse strain may be useful in studies of Bloom Syndrome.
A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted upstream of exon 8. This construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase plasmid to remove the selection cassette. ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exon 8 were injected in recipient blastocysts. Resulting chimeric male animals were backcrossed to NIH Black Swiss outbred mice. Upon arrival at The Jackson Laboratory the mice were crossed with C57BL/6J once to establish the colony.
Allele Name | targeted mutation 4, Nicholas Chester |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Blmflox |
Gene Symbol and Name | Blm, Bloom syndrome, RecQ like helicase |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 7 |
Molecular Note | A floxed PGK-neomycin resistance gene was inserted into intron 8 and an additional loxP site was placed in intron 7, 75 bp from the start of exon 8. Transient cre expression removed the PGK-neo sequence from correctly targeted ES cells, leaving loxP sites flanking exon 8. |
Mutations Made By | Dr. Philip Leder, Harvard Medical School |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the STOCK Blmtm4Ches/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008670 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Blm<tm4Ches> |
Frozen Mouse Embryo | STOCK Blm<tm4Ches>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Blm<tm4Ches>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Blm<tm4Ches>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Blm<tm4Ches>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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