Fmn pro-delta/pro-delta mice (Fmn1, formin 1) do not express any of the major splice variants of Fmn1. Homozygotes display digital syndactyly phenotype and unilateral renal aplasia. This mutant mouse strain may be useful in studies of limb development, digital syndactyly, and kidney development.
Dr. Philip Leder, Harvard Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fmn1 | formin 1 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. None of the major splice variants of Fmn1 are detected by Northern blot analysis. No gene product (protein) is detected by Western blot analysis of MEFs. Homozygotes display digital oligodactyly phenotype, with absent fibula. 50% of homozygotes (on the FVB background) exhibit unilateral renal aplasia. This mutant mouse strain may be useful in studies of limb development, digital oligodactyly, and kidney development.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 9. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. The resulting chimeric animals were crossed to 129/SvEv mice, and then backcrossed to FVB for 10 generations before arriving at The Jackson Laboratory. The mice were then crossed to FVB/NJ once.
Allele Name | targeted mutation 2, Markus Dettenhofer |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Fmn1Pro |
Gene Symbol and Name | Fmn1, formin 1 |
Gene Synonym(s) | |
Promoter | Fmn1, formin 1, mouse, laboratory |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | A neo cassette was inserted into exon 9. This insertion leads to a disruption at the proline-rich FH1 domain. The absence of protein was confirmed by western blot analysis of embryonic fibroblasts extracts. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the FVB.129S6-Fmn1tm2Made/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008665 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Fmn1<tm3Led> |
Frozen Mouse Embryo | FVB.129S6-Fmn1<tm2Made>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.129S6-Fmn1<tm2Made>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.129S6-Fmn1<tm2Made>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB.129S6-Fmn1<tm2Made>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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