FVB.129S6-Fmn1^tm2Made/J

Stock number: 008665
Strain synonyms: FVB.129S6-Fmn1^tm3Led/J
Research areas: Developmental Biology Research, Internal/Organ Research

Description

Fmn pro-delta/pro-delta mice (Fmn1, formin 1) do not express any of the major splice variants of Fmn1. Homozygotes display digital syndactyly phenotype and unilateral renal aplasia. This mutant mouse strain may be useful in studies of limb development, digital syndactyly, and kidney development.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. None of the major splice variants of Fmn1 are detected by Northern blot analysis. No gene product (protein) is detected by Western blot analysis of MEFs. Homozygotes display digital oligodactyly phenotype, with absent fibula. 50% of homozygotes (on the FVB background) exhibit unilateral renal aplasia. This mutant mouse strain may be useful in studies of limb development, digital oligodactyly, and kidney development.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 9. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. The resulting chimeric animals were crossed to 129/SvEv mice, and then backcrossed to FVB for 10 generations before arriving at The Jackson Laboratory. The mice were then crossed to FVB/NJ once.

Allele

Symbol: Fmn1^tm2Made
Name: targeted mutation 2, Markus Dettenhofer
MGI accession ID: MGI:3848983
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Fmn1
Marker name: formin 1
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: A neo cassette was inserted into exon 9. This insertion leads to a disruption at the proline-rich FH1 domain. The absence of protein was confirmed by western blot analysis of embryonic fibroblasts extracts.