129S-Trp53^tm3Tyj/J

Stock number: 008651
Product name: p53^LSL-R270H
Strain synonyms: p53LSL.R270H 129svj
Research areas: Apoptosis Research, Cancer Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These mice carry a conditional point-mutant allele of the transformation related protein 53 gene (p53R270H; contact mutant homologous to human p53R273H). The conditional allele is functionally equivalent to a null mutation. Mice have all the phenotypic issues of p53 knockout mice and therefore decreased viability of homozygotes is observed. Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

Detailed description

These mice carry a conditional point-mutant allele of the transformation related protein 53 gene (p53R270H; contact mutant homologous to human p53R273H). The conditional allele is functionally equivalent to a null mutation. Mice have all the phenotypic issues of p53 knockout mice and therefore have some decreased viability of homozygotes. Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

Development

A targeting vector was designed to place a loxP-flanked stop cassette in intron 1 and an R270H missense mutation into exon 8. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. The strain was maintained on a 129S4 background by the donating laboratory.

Allele

Symbol: Trp53^tm3Tyj
Name: targeted mutation 3, Tyler Jacks
MGI accession ID: MGI:3039265
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Trp53
Marker name: transformation related protein 53
Chromosome: 11
Strain of origin: 129S4/SvJae
Molecular note: A targeting construct was designed to insert a loxP flanked stop cassette into intron 1 and an R270H missense mutation into exon 8.