B6.129S1-Kif5b^tm1Njen/J

Stock number: 008637
Research areas: Cell Biology Research, Research Tools

Description

These mice possess loxP sites on either side of exon 2 in the kinesin family member 5B gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene to elucidate the gene's function.

Detailed description

These mice possess loxP sites on either side of exon 2 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene to elucidate the gene's function.

For example, when crossed to a strain expressing Cre recombinase in pancreatic beta cells (see Stock No. 003573), this mutant mouse strain may be useful in studies of beta cell development and insulin secretion.

Development

Exon 2 was flanked by loxP sites using 129S1/Sv-p⁺Tyr⁺Kitl ⁺-derived CJ7 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for more than 6 generations by the donating laboratory.

Allele

Symbol: Kif5b^tm1Njen
Name: targeted mutation 1, Nancy A Jenkins
MGI accession ID: MGI:3809821
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Kif5B^fl; Kinesin-1B II
Marker symbol: Kif5b
Marker name: kinesin family member 5B
Marker synonyms: HEL-S-61; Khc; kinesin heavy chain; KINH; KNS; KNS1; UKHC
Chromosome: 18
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: Exon 2 is flanked by loxP sites. An FRT-flanked neo cassette was removed by flp-mediated recombination.