These mice possess loxP sites on either side of exon 2 in the kinesin family member 5B gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene to elucidate the gene's function.
Dr. Lawrence S.B. Goldstein, UCSD
These mice possess loxP sites on either side of exon 2 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene to elucidate the gene's function.
For example, when crossed to a strain expressing Cre recombinase in pancreatic beta cells (see Stock No. 003573), this mutant mouse strain may be useful in studies of beta cell development and insulin secretion.
Exon 2 was flanked by loxP sites using 129S1/Sv-p+Tyr+Kitl +-derived CJ7 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for more than 6 generations by the donating laboratory.
|Allele Name||targeted mutation 1, Nancy A Jenkins|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Kif5Bfl; Kinesin-1B II|
|Gene Symbol and Name||Kif5b, kinesin family member 5B|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||Exon 2 is flanked by loxP sites. An FRT-flanked neo cassette was removed by flp-mediated recombination.|
|Mutations Made By|| |
Dr. Nancy Jenkins, Institute of Molecular and Cell Biology
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129S1-Kif5btm1Njen/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008637 in your Materials and Methods section.