These mice possess loxP sites on either side of exon 2 in the kinesin family member 5B gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene to elucidate the gene's function.
Dr. Lawrence S.B. Goldstein, UCSD
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Kif5b | kinesin family member 5B |
These mice possess loxP sites on either side of exon 2 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene to elucidate the gene's function.
For example, when crossed to a strain expressing Cre recombinase in pancreatic beta cells (see Stock No. 003573), this mutant mouse strain may be useful in studies of beta cell development and insulin secretion.
Exon 2 was flanked by loxP sites using 129S1/Sv-p+Tyr+Kitl +-derived CJ7 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for more than 6 generations by the donating laboratory.
Allele Name | targeted mutation 1, Nancy A Jenkins |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Kif5Bfl; Kinesin-1B II |
Gene Symbol and Name | Kif5b, kinesin family member 5B |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 18 |
Molecular Note | Exon 2 is flanked by loxP sites. An FRT-flanked neo cassette was removed by flp-mediated recombination. |
Mutations Made By | Dr. Nancy Jenkins, Institute of Molecular and Cell Biology |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129S1-Kif5btm1Njen/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008637 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Kif5b<tm1Njen> |
Frozen Mouse Embryo | B6.129S1-Kif5b<tm1Njen>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Kif5b<tm1Njen>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Kif5b<tm1Njen>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Kif5b<tm1Njen>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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