Loss of MAVS (mitochondrial antiviral signaling) protein expression in this knock-out strain abolishes viral induction of interferons and prevents the activation of NFΚB and IRF3 in multiple cell types, except plasmacytoid dendritic cells. Mice lacking the protein fail to induce interferons in response to poly(I:C) stimulation and are severely compromised in immune defense against viral infection.
Zhijian 'James' Chen, UT Southwestern
Genetic Background | Generation |
---|---|
F?+F20
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Mavs | mitochondrial antiviral signaling protein |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Loss of MAVS (mitochondrial antiviral signaling) protein expression abolishes viral induction of interferons and prevents the activation of NFΚB (Rela, v-rel reticuloendotheliosis viral oncogene homolog A (avian)) and IRF3 (interferon regulatory factor 3) in multiple cell types, except plasmacytoid dendritic cells (pDCs). The protein is not required for interferon induction by cytosolic DNA or by Listeria monocytogenes. Mice lacking the protein fail to induce interferons in response to poly(I:C) stimulation and are severely compromised in immune defense against viral infection. Experimental results provide in vivo evidence that the cytosolic viral signaling pathway through the targeted gene is specifically required for innate immune responses against viral infection.
A segment of exon 2 (starting at the ATG start codon) and exon 3 were replaced with a PGK-neomycin resistance cassette flanked by loxP sites. A 129-derived embryonic stem cell line was used to create the mutation. Germline chimaera's were bred to C57BL/6. This strain was maintained on a mixed C57BL/6 and 129SvEv genetic background by the donating laboratory.
Allele Name | targeted mutation 1, Zhijian J Chen |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Mavs- |
Gene Symbol and Name | Mavs, mitochondrial antiviral signaling protein |
Gene Synonym(s) | |
Strain of Origin | Not Specified |
Chromosome | 2 |
Molecular Note | Sequence beginning at the ATG in exon 2 and extending through exon 3 was replaced with a floxed neomycin resistance gene. Immunoblot failed to detect protein in mutants. |
Mutations Made By | Zhijian 'James' Chen, UT Southwestern |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the Mavs- mouse strain in a publication, please cite the originating article(s) and include JAX stock #008634 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype Mavs<tm1Zjc> |
Frozen Mouse Embryo | B6;129-Mavs<tm1Zjc>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Mavs<tm1Zjc>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Mavs<tm1Zjc>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Mavs<tm1Zjc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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