B6.BXD8-Klra8^Cmv1-del/WumJ

Stock number: 008633
Research areas: Immunology, Inflammation and Autoimmunity Research, Virology Research

Description

Mice of this strain harbor a deletion in the killer cell lectin-like receptor, subfamily A, member 8; cytomegalovirus resistance 1 gene (Klra8, originally Ly49h).

Detailed description

Animals lacking Ly49H (Klra8, killer cell lectin-like receptor, subfamily A, member 8) expression on natural killer cells were selected from progeny of BXD8 recombinant inbred mice backcrossed to C57BL/6 for ten generations. A number of other intact killer cell lectin-like receptors and integrin alpha M (Mac1) are still effectively expressed on natural killer cells. Natural killer cell functions (e.g. upregulation of IFN-gamma after activation via NK1.1, and cellular proliferation) are unaffected by this mutation. Compared to the parental C57BL/6 strain, these mutant mice are more susceptible to murine cytomegalovirus (MCMV), but not herpes simplex virus-1 (HSV-1) or ectromelia virus infection. Homozygotes are viable and fertile.

Development

The recombinant inbred strain BXD8 was backcrossed to C57BL/6NCr for ten generations, selecting for animals lacking Ly49H (Klra8, killer cell lectin-like receptor, subfamily A, member 8) expression on natural killer cells. Cell surface staining with anti-Ly49H-specific antibody 3D10 was used to follow the marker. Analysis of 110 polymorphic microsatellite markers by the donating laboratory indicated that the original BXD8 recombinant inbred strain carried 52% of C57BL/6-derived genome; the congenic demonstrated 100% of C57BL/6-derived genome, with the exception of Ly49H.

Allele

Symbol: Klra8^Cmv1-del
Name: cytomegalovirus resistance 1, deletion
MGI accession ID: MGI:3773165
Generation method: Not Applicable
Marker symbol: Klra8
Marker name: killer cell lectin-like receptor, subfamily A, member 8
Chromosome: 6
Strain of origin: BXD8/TyJ
Molecular note: Mapping experiments identified a 23 kb deletion in the Klra cluster in the BXD-8/Ty strain that contained only the Klra8 gene. This deletion correlated with an increased susceptiblity to cytomegalovirus infection.