Mice of this strain harbor a deletion in the killer cell lectin-like receptor, subfamily A, member 8; cytomegalovirus resistance 1 gene (Klra8, originally Ly49h).
Wayne Yokoyama, Washington University Sch. Med., HHMI
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Not Applicable | Klra8 | killer cell lectin-like receptor, subfamily A, member 8 |
Animals lacking Ly49H (Klra8, killer cell lectin-like receptor, subfamily A, member 8) expression on natural killer cells were selected from progeny of BXD8 recombinant inbred mice backcrossed to C57BL/6 for ten generations. A number of other intact killer cell lectin-like receptors and integrin alpha M (Mac1) are still effectively expressed on natural killer cells. Natural killer cell functions (e.g. upregulation of IFN-gamma after activation via NK1.1, and cellular proliferation) are unaffected by this mutation. Compared to the parental C57BL/6 strain, these mutant mice are more susceptible to murine cytomegalovirus (MCMV), but not herpes simplex virus-1 (HSV-1) or ectromelia virus infection. Homozygotes are viable and fertile.
The recombinant inbred strain BXD8 was backcrossed to C57BL/6NCr for ten generations, selecting for animals lacking Ly49H (Klra8, killer cell lectin-like receptor, subfamily A, member 8) expression on natural killer cells. Cell surface staining with anti-Ly49H-specific antibody 3D10 was used to follow the marker. Analysis of 110 polymorphic microsatellite markers by the donating laboratory indicated that the original BXD8 recombinant inbred strain carried 52% of C57BL/6-derived genome; the congenic demonstrated 100% of C57BL/6-derived genome, with the exception of Ly49H.
Allele Name | cytomegalovirus resistance 1, deletion |
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Allele Type | Not Applicable |
Allele Synonym(s) | Klra8KO; L49h- |
Gene Symbol and Name | Klra8, killer cell lectin-like receptor, subfamily A, member 8 |
Gene Synonym(s) | |
Strain of Origin | BXD8/TyJ |
Chromosome | 6 |
Molecular Note | Mapping experiments identified a 23 kb deletion in the Klra cluster in the BXD-8/Ty strain that contained only the Klra8 gene. This deletion correlated with an increased susceptiblity to cytomegalovirus infection. |
When maintained as a live colony, homozygotes may be bred.
When using the B6.BXD8-Klra8Cmv1-del/WumJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #008633 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Klra8<Cmv1-del> |
Frozen Mouse Embryo | B6.BXD8-Klra8<Cmv1-del>/WumJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.BXD8-Klra8<Cmv1-del>/WumJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.BXD8-Klra8<Cmv1-del>/WumJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.BXD8-Klra8<Cmv1-del>/WumJ Frozen Embryo | $3373.50 |
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