Homozygous kinesin family member 5B targeted mutant mice die early during embryonic developmnent (before 9.5 dpc). Heterozygotes do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of intracellular transport in neurons and other cell types.
Dr. Lawrence S.B. Goldstein, UCSD
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Kif5b | kinesin family member 5B |
Homozygous targeted mutant mice die early during embryonic developmnent (before 9.5 dpc). Heterozygotes are viable, may have reduced fertility, are normal in size, and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of intracellular transport in neurons and other cell types.
Exon 2 was flanked by loxP sites using 129S1/Sv-p+Tyr+Kitl +-derived CJ7 embryonic stem (ES) cells. The floxed fragment was excised with beta actin promoter Cre, leaving a single loxP site. This line was backcrossed to C57BL/6 for more than 10 generations by the donating laboratory.
Allele Name | targeted mutation 1.1, Nancy A Jenkins |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Kif5BI; Kif5Bnull; Kinesin-1B I |
Gene Symbol and Name | Kif5b, kinesin family member 5B |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 18 |
Molecular Note | Exon 2 was flanked by loxP sites creating the Kif5btm1Njen allele. The floxed fragment was excised with a beta actin promoter-driven Cre, leaving a single loxP site. |
Mutations Made By | Dr. Nancy Jenkins, Institute of Molecular and Cell Biology |
When maintained as a live colony, heterozygotes may be bred. Homozygotes are embryonic lethal.
When using the B6.129S1-Kif5btm1.1Njen/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008632 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Kif5b<tm1.1Njen> |
Frozen Mouse Embryo | B6.129S1-Kif5b<tm1.1Njen>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Kif5b<tm1.1Njen>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Kif5b<tm1.1Njen>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Kif5b<tm1.1Njen>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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