B6.129S1-Kif5b^tm1.1Njen/J

Stock number: 008632
Research areas: Cell Biology Research, Developmental Biology Research

Description

Homozygous kinesin family member 5B targeted mutant mice die early during embryonic developmnent (before 9.5 dpc). Heterozygotes do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of intracellular transport in neurons and other cell types.

Detailed description

Homozygous targeted mutant mice die early during embryonic developmnent (before 9.5 dpc). Heterozygotes are viable, may have reduced fertility, are normal in size, and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of intracellular transport in neurons and other cell types.

Development

Exon 2 was flanked by loxP sites using 129S1/Sv-p⁺Tyr⁺Kitl ⁺-derived CJ7 embryonic stem (ES) cells. The floxed fragment was excised with beta actin promoter Cre, leaving a single loxP site. This line was backcrossed to C57BL/6 for more than 10 generations by the donating laboratory.

Allele

Symbol: Kif5b^tm1.1Njen
Name: targeted mutation 1.1, Nancy A Jenkins
MGI accession ID: MGI:3810072
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: Kif5B^I; Kif5B^null; Kinesin-1B I
Marker symbol: Kif5b
Marker name: kinesin family member 5B
Marker synonyms: HEL-S-61; Khc; kinesin heavy chain; KINH; KNS; KNS1; UKHC
Chromosome: 18
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: Exon 2 was flanked by loxP sites creating the Kif5b^tm1Njen allele. The floxed fragment was excised with a beta actin promoter-driven Cre, leaving a single loxP site.