Mice homozygous for this spontaneous mutation of the calcium channel, voltage-dependent, beta 4 subunit gene exhibit a lethargic behavior with gait instability and the occasional seizure. These mice may be useful in studies of epilepsy and voltage-dependent calcium channels.Read More +
Mice homozygous for the spontaneous mutation lethargic 2J have a similar phenotype to lethargic mice (See 000504). They exhibit lethargic behavior with gait instability and the occasional seizure. The molecular mutation is unknown, however, no CACNB4 protein product is detected by Western blot.
This spontaneous mutation arose on DBA/2J in the production colonies of The Jackson Laboratory and was backcrossed a minimum of five generations to C57BL/6J in the laboratory of Dr. Wayne Frankel and Dr. Verity Letts. In an effort to improve breeding performance, it was subsequently crossed to B6C3FeF1/J a/a, intercrossed to produce homozygotes and then maintained as a sibling mating. The strain was donated to the Repository in 2008.
|Allele Name||lethargic 2J|
|Gene Symbol and Name||Cacnb4, calcium channel, voltage-dependent, beta 4 subunit|
|Strain of Origin||DBA/2J|
|Molecular Note||Mutation in the gene is unknown, but no protein product is detected by Western blot.|
On a mixed background this strain can be maintained by homozygous matings. On a C57BL/6J background, the strain must be maintained by heterozygote x heterozygote matings.
When using the STOCK Cacnb4lh-2J/LetJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #008626 in your Materials and Methods section.