STOCK Cacnb4^lh-2J/LetJ

Stock number: 008626
Research areas: Cell Biology Research, Neurobiology Research

Description

Mice homozygous for this spontaneous mutation of the calcium channel, voltage-dependent, beta 4 subunit gene exhibit a lethargic behavior with gait instability and the occasional seizure. These mice may be useful in studies of epilepsy and voltage-dependent calcium channels.

Detailed description

Mice homozygous for the spontaneous mutation lethargic 2J have a similar phenotype to lethargic mice (See 000504). They exhibit lethargic behavior with gait instability and the occasional seizure. The molecular mutation is unknown, however, no CACNB4 protein product is detected by Western blot.

Development

This spontaneous mutation arose on DBA/2J in the production colonies of The Jackson Laboratory and was backcrossed a minimum of five generations to C57BL/6J in the laboratory of Dr. Wayne Frankel and Dr. Verity Letts. In an effort to improve breeding performance, it was subsequently crossed to B6C3FeF1/J a/a, intercrossed to produce homozygotes and then maintained as a sibling mating. The strain was donated to the Repository in 2008.

Allele

Symbol: Cacnb4^lh-2J
Name: lethargic 2J
MGI accession ID: MGI:2155890
Generation method: Spontaneous
Marker symbol: Cacnb4
Marker name: calcium channel, voltage-dependent, beta 4 subunit
Chromosome: 2
Strain of origin: DBA/2J
Molecular note: Mutation in the gene is unknown, but no protein product is detected by Western blot.