B6;129-Fzd5^tm1Nat/J

Stock number: 008621
Product name: Fz5 lacZ KO
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools, Sensorineural Research

Description

These frizzled 5 targeted mutant mice carry a beta galactosidase reporter. Heterozygous embryos show lacZ expression in the neural ridge at the anterior of the neural plate at E8.5, in the telencephalon, optic vesicle, hindgut and midgut at E10.5, and in the retinas at E16.5. Six-week-old animals express lacZ in the parafascicular nucleus and hypothalamus. Homozygous mice have placenta and yolk sac defects, thus die midway through gestation.

Detailed description

These frizzled 5 targeted mutant mice carry a beta galactosidase reporter. Heterozygous embryos show lacZ expression in the neural ridge at the anterior of the neural plate at E8.5, in the telencephalon, optic vesicle, hindgut and midgut at E10.5, and in the retinas at E16.5. Six-week-old animals express lacZ in the parafascicular nucleus and hypothalamus. Homozygous mice have placenta and yolk sac defects, thus die midway through gestation. In situ hybridization has confirmed that transcripts from the targeted gene are absent.

Development

Exon 2, which contains the entire coding region, was replaced with a beta galactosidase reporter followed by a PGK-neo selection cassette. The mutation was created using a 129-derived embryonic stem (ES) cell line and maintained on a mixed C57BL/6 and 129 background by the donating laboratory.

Allele

Symbol: Fzd5^tm1Nat
Name: targeted mutation 1, Jeremy Nathans
MGI accession ID: MGI:3796576
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Synonyms: Fz5^lacZ
Marker symbol: Fzd5
Marker name: frizzled class receptor 5
Marker synonyms: 5330434N09Rik; C2orf31; Fz5; HFZ5; MCOPCB11
Chromosome: 1
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: LacZ expression is found in the neural ridge at the anterior of the neural plate at E8.5, in the telencephalon, optic vesicle, hindgut and midgut at E10.5, and in the retinas at E16.5. Six-week-old animals express lacZ in the parafascicular nucleus and hypothalamus.
Molecular note: Exon 2, which contains the entire coding region, was replaced with a lacZ/neo cassette. This is a null allele and a reporter allele.