B6;129-Fzd5^tm2Nat/J

Stock number: 008620
Product name: Fz5 CKO-AP
Research areas: Neurobiology Research, Research Tools, Sensorineural Research

Description

This is a conditional allele of the frizzled 5 gene in which the coding region was flanked by loxP sites and an alkaline phosphatase (AP) reporter was placed downstream. Cre-mediated recombination eliminates the coding region in a tissue-specific manner, ablates expression of the gene, and causes alkaline phosphatase (AP) to be expressed under the control of the frizzled 5 promoter. If recombination is mediated by an embryonic tissue-specific Cre, such as Sox2-Cre (e.g. Stock No. 004783), mice show a loss of cells in the parafasicular nucleus of the thalamus and defects in eye development.

Detailed description

This is a conditional allele of the frizzled 5 gene in which the coding region was flanked by loxP sites and an alkaline phosphatase (AP) reporter was placed downstream. Cre-mediated recombination eliminates the coding region in a tissue-specific manner, ablates expression of the gene, and causes alkaline phosphatase (AP) to be expressed under the control of the frizzled 5 promoter.

If both floxed alleles are excised, mutants are embryonic lethal at day 10 due to a placental defect. If recombination is mediated by an embryonic tissue-specific Cre, such as Sox2-Cre (e.g. Stock No's. 004783, 008454), mice show a loss of cells in the parafasicular nucleus (PFN) of the thalamus and defects in eye development.

When bred to a strain expressing a tamoxifen inducible Cre recombinase (see Stock No. 004847 for example), this mutant mouse strain exhibits a loss of AP expressing neurons in the PFN of the thymus.

Development

A Hind III-tagged loxP site was introduced to exon 2, 5' of the initiator methionine codon, and a second loxP site, alkaline phosphatase (AP) open reading frame, and an FRT-flanked PGK-neomycin cassette were placed in intron 2 via homologous recombination in 129-derived embryonic stem cells. The neomycin cassette was excised through crosses with an Flp recombinase-expressing strain, leaving exon 2 flanked by loxP sites upstream of AP. This line was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.

Allele

Symbol: Fzd5^tm2Nat
Name: targeted mutation 2, Jeremy Nathans
MGI accession ID: MGI:3796577
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Fzd5
Marker name: frizzled class receptor 5
Chromosome: 1
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A loxP site was inserted downstream of exon 1 and a cassette containg a 5' loxP site, alkaline phosphatase gene and an frt-flanked neo gene was inserted downstream of exon 2. Germ line, flp-mediated recombination was used to remove the neo cassette leaving exon 2 floxed and the alkaline phosphatase gene intact. This is a floxed allele and a reporter allele.