Overview

Also Known As:Vav-iCre

Vav-iCre transgenic mice have the mouse HS21/45 control regions directing expression of an optimized variant of Cre recombinase (iCre) to hematopoietic cells (and their progenitors), and may be useful for generating conditional mutations in hematopoietic cells.

In Goodwin et al. 2019 Genome Res. 29:494, it was discovered that the transgene integrated into an intron of Commd10 (COMM domain containing 10) on chromosome 18 - creating a Commd10 null allele.

Donating Investigator

Dimitris Kioussis, MRC Natl Inst for Med Research

Genetic overview

Genetic Background	Generation
	N16+pN2F2 (2018-10-29 00:00:00)

Commd10^{Tg(Vav1-icre)A2Kio}

Allele Type
Transgenic (Recombinase-expressing, Null/Knockout)

View Genetics

Research Applications

Hematological Research
Immunology, Inflammation and Autoimmunity Research
Cancer Research
Research Tools
Developmental Biology Research

View All Research Applications

Base Price

Starting at:

$255.00 Domestic price for female 4-week

333.51 Domestic price for breeder pair

View Price List

Details

Detailed Description

Vav-iCre transgenic mice express an improved Cre recombinase (iCre) under the control of the mouse vav 1 oncogene (Vav1) promoter. The transgene integrated into an intron of Commd10 (COMM domain containing 10) on chromosome 18. The insertion results in a functional knock-out of Commd10 in homozygous mice.

Mice hemizygous for this Vav-iCre transgene are viable and fertile, with the mouse HS21/45-vav control regions directing expression of an optimized variant of Cre recombinase (iCre) to hematopoietic cells (and their progenitors). Using crosses to a reporter strain, variegated germ line (testis and ovaries), and heart and gut expression is also reported. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Vav-iCre transgenic mice may be useful for generating conditional mutations in hematopoietic cells.

Joseph et al. 2013 Cell Stem Cell 13:520 reports that Vav-iCre mice express iCre in hematopoietic cells and endothelial cells, as well as in the testes. For Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Vav-iCre females with floxed males. However, female germline expression may also be observed (see below).

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Commd10^{Tg(Vav1-icre)A2Kio}). This same information would also be found searching the MGI Recombinase Activity database.

This Vav-iCre strain (Stock No. 008610) allows reliable deletion of specific genes throughout the entire hematopoietic compartment, whereas the hCD2-iCre strain (Stock No. 008520) allows targeting to be focused to T cells and B cells.

Development

The Vav-iCre transgene was designed with the mouse vav 1 oncogene (Vav1) promoter region (containing the 2 proximal upstream DNase-I hypersensitive (HS) sites (HS21)), an optimized variant of Cre recombinase (iCre; improved with mammalian codon usage, removed putative cryptic splce sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals) and an SV40 polyA signal replacing Vav1 exon 1, and Vav1 intron 1 (containing both proximal HS sites (HS45)). The transgene was microinjected into the pronuclei of fertilized oocytes from (CBA/Ca x C57BL/10)F1 mice. Founder mice (founder line ".A2") were established and used to generate mutant mice. The resulting Vav-iCre transgenic mice were subsequently bred to wildtype C57BL/6 mice for more than 16 generations prior to arrival at The Jackson Laboratory Repository. Upon arrival, the Vav-iCre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony. As of 2015, SNP testing mice from several pedigree lines showed all 5 markers determining C57BL/6 substrain to be C57BL/6J allele-type.
In 2018, it was discovered that the transgene integrated into an intron of Commd10 (COMM domain containing 10) on chromosome 18 - creating a Commd10 null allele.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase is expressed in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches, ovaries and testes.

Control Suggestions

Noncarrier

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

De Boer J; Williams A; Skavdis G; Harker N; Coles M; Tolaini M; Norton T; Williams K; Roderick K; Potocnik AJ; Kioussis D. 2003. Transgenic mice with hematopoietic and lymphoid specific expression of Cre. Eur J Immunol 33(2):314-25PubMed: 12548562 MGI: J:81568
Ogilvy S; Elefanty AG; Visvader J; Bath ML; Harris AW; Adams JM. 1998. Transcriptional regulation of vav, a gene expressed throughout the hematopoietic compartment. Blood 91(2):419-30PubMed: 9427694 MGI: J:45594
Ogilvy S; Metcalf D; Gibson L; Bath ML; Harris AW; Adams JM. 1999. Promoter elements of vav drive transgene expression in vivo throughout the hematopoietic compartment. Blood 94(6):1855-63PubMed: 10477714 MGI: J:140234
Shimshek DR; Kim J; Hubner MR; Spergel DJ; Buchholz F; Casanova E; Stewart AF; Seeburg PH; Sprengel R. 2002. Codon-improved Cre recombinase (iCre) expression in the mouse. Genesis 32(1):19-26PubMed: 11835670 MGI: J:78663

View All References

Genetics

Commd10^{Tg(Vav1-icre)A2Kio}

Allele Symbol: Commd10^{Tg(Vav1-icre)A2Kio}

Allele Name	transgene insertion A2, Dimitris Kioussis
Allele Type	Transgenic (Recombinase-expressing, Null/Knockout)
Allele Synonym(s)	Tg(Vav1-cre)1Kio; Tg(Vav1-cre)A2Kio; Tg(Vav1-icre)A2Kio; Vav1 (HS21/5)-iCre; Vav1-Cre; VavCre; Vav-cre; VavCre^deBoer; Vav-iCre
Gene Symbol and Name	Commd10, COMM domain containing 10
Gene Synonym(s)
Promoter	Vav1, vav 1 oncogene, mouse, laboratory
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase is expressed in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches, ovaries and testes.
Strain of Origin	(CBA/Ca x C57BL/10)F2
Chromosome	18
Molecular Note	This transgene expresses Cre recombinase under the control of a vav promoter. This promoter was shown to be active in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches, ovaries and testes. Line A2 inserted into the gene at 47022629-47022630 (Build GRCm38/mm10). The insetion results in a functional knock-out in homozygous mice.
Mutations Made By	Dimitris Kioussis, MRC Natl Inst for Med Research

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Hematological Research
- Hematopoietic Defects
Immunology, Inflammation and Autoimmunity Research
- Lymphoid Tissue Defects
  - hematopoietic development
Cancer Research
- Cre-lox System
  - Cre recombinase expression
Research Tools
- Cancer Research
  - Cre-lox System
- Cre-lox System
  - Cre Recombinase Expression
- Genetics Research
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis: Cre-lox System
- Developmental Biology Research
  - Cre-lox System
- Immunology, Inflammation and Autoimmunity Research
- Hematological Research
Developmental Biology Research
- Internal/Organ Defects
  - hematopoietic defects
- Lymphoid Tissue Defects
  - hematopoietic defects

Mammalian Phenotype Terms by Genotype

References

De Boer J; Williams A; Skavdis G; Harker N; Coles M; Tolaini M; Norton T; Williams K; Roderick K; Potocnik AJ; Kioussis D. 2003. Transgenic mice with hematopoietic and lymphoid specific expression of Cre. Eur J Immunol 33(2):314-25PubMed: 12548562 MGI: J:81568
Ogilvy S; Elefanty AG; Visvader J; Bath ML; Harris AW; Adams JM. 1998. Transcriptional regulation of vav, a gene expressed throughout the hematopoietic compartment. Blood 91(2):419-30PubMed: 9427694 MGI: J:45594
Ogilvy S; Metcalf D; Gibson L; Bath ML; Harris AW; Adams JM. 1999. Promoter elements of vav drive transgene expression in vivo throughout the hematopoietic compartment. Blood 94(6):1855-63PubMed: 10477714 MGI: J:140234
Shimshek DR; Kim J; Hubner MR; Spergel DJ; Buchholz F; Casanova E; Stewart AF; Seeburg PH; Sprengel R. 2002. Codon-improved Cre recombinase (iCre) expression in the mouse. Genesis 32(1):19-26PubMed: 11835670 MGI: J:78663

Additional - Commd10^{Tg(Vav1-icre)A2Kio} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Generic iCre
- Standard PCR:Tg(Vav1-cre)A2Kio
- Standard PCR:Generic iCre
- Probe:Generic iCre Probe
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57BL/6J. The donating investigator reports that homozygous mice are not viable.
Joseph et al. 2013 Cell Stem Cell 13:520 reports that Vav-iCre mice express iCre in hematopoietic cells and endothelial cells, as well as in the testes. For Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding Vav-iCre females with floxed males. However, female germline expression may also be observed (see the Mouse Genome Informatics (MGI) Allele Detail entry: Commd10^{Tg(Vav1-icre)A2Kio}).
- Additional Breeding and Husbandry Support
Mating System
- Noncarrier x Hemizygote
Citation
When using the Vav-iCre mouse strain in a publication, please cite the originating article(s) and include JAX stock #008610 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX18 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Hemizygous or non-carrier for Tg(Vav1-cre)A2Kio

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Vav-iCre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Commd10Tg(Vav1-icre)A2Kio

Allele Symbol: Commd10Tg(Vav1-icre)A2Kio

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Commd10Tg(Vav1-icre)A2Kio related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Commd10^{Tg(Vav1-icre)A2Kio}

Allele Symbol: Commd10^{Tg(Vav1-icre)A2Kio}

Additional - Commd10^{Tg(Vav1-icre)A2Kio} related