These mice have a targeted "knockout" mutation of the Dmc1 gene (DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)) and may be useful in studying gametogenesis and meiosis, including double strand breaks, chromosome asynapsis, DNA repair mechanisms, homologous recombination, and the pachytene checkpoint.
John Schimenti, Cornell University
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Dmc1 | DNA meiotic recombinase 1 |
While mice heterozygous for this meiosis-specific RecA homolog (Dmc1) mutation are viable and fertile, homozygotes are viable but sterile due to arrest of gametogenesis in the first meiotic prophase (prophase I). No RNA message from the targeted gene is observed in homozygous testis. Homozygous males have reduced testis size and no mature spermatozoa in the epididymis; spermatocytes undergo meiotic arrest from defective double strand break repair and extensive chromosome asynapsis. Homozygous females exhibit defective oogenesis due to similar aberrations occurring at the pachytene stage or earlier and thus have small, malformed ovaries with complete depletion of oocytes and follicles by adulthood. These Dmc1-mutant mice may be useful in studying gametogenesis and meiosis (including double strand breaks, chromosome asynapsis, DNA repair mechanisms, homologous recombination, and the pachytene checkpoint).
A targeting vector was designed to replace a region of the targeted gene containing the RecA-like DNA binding motif (GEFRTGKT) with a neomycin resistance cassette. This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females. Mutant mice were backcrossed to C57BL/6J for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, John C Schimenti |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Dmc1- |
Gene Symbol and Name | Dmc1, DNA meiotic recombinase 1 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 15 |
Molecular Note | A neomycin selection cassette replaced a genomic fragment containing sequences encoding the conserved DNA binding motif required for protein activity. RT-PCR analysis on RNA derived from testis of homozygous males demonstrated that no detectable transcript was produced from this allele. |
Mutations Made By | John Schimenti, Cornell University |
When maintaining a live colony, heterozygous mice may be bred together (or to wildtype siblings or C57BL/6J inbred mice). Homozygous mice are viable but sterile.
When using the B6.Cg-Dmc1tm1Jcs/JcsJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #008608 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Dmc1<tm1Jcs> |
Frozen Mouse Embryo | B6.Cg-Dmc1<tm1Jcs>/JcsJ | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Dmc1<tm1Jcs>/JcsJ | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Dmc1<tm1Jcs>/JcsJ | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Dmc1<tm1Jcs>/JcsJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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