Mice homozygous for this Galp (galanin-like peptide) targeted mutation gain less weight than wildtype controls when fed a high fat diet. This mutant mouse strain may be useful in studies of energy homeostasis, nutrition and obesity.
Robert A. Steiner, University of Washington, Box 357290
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Galp | galanin-like peptide |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by RT-PCR analysis. Homozygotes are indistinguishable from wildtype when maintained on ad libitum diet. After fasting, male homozygotes consume less food than wildtype controls. When fed a high fat diet homozygotes gain less weight than wildtype controls. This mutant mouse strain may be useful in studies of energy homeostasis, nutrition and obesity.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 2 (contains the translation start site). The construct was electroporated into 129 derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts generated by mating C57BL/6 and B6C3 mice. to C57BL/6 mice to generate the founders, and then crossed to C57BL/6 and C3H mice.
Allele Name | targeted mutation 1, Robert A Steiner |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Galp, galanin-like peptide |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 7 |
Molecular Note | Exon 2 was replaced with a neo cassette. The absence of transcript expression was confirmed by RT-PCR analysis. |
Mutations Made By | Robert Steiner, University of Washington, Box 357290 |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the STOCK Galptm1Stei/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008598 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Galp<tm1Stei> |
Frozen Mouse Embryo | STOCK Galp<tm1Stei>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Galp<tm1Stei>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Galp<tm1Stei>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Galp<tm1Stei>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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