STOCK Galp^tm1Stei/J

Stock number: 008598
Strain synonyms: B6;C3(129)-Galp<tm1Stei>/J
Research areas: Diabetes and Obesity Research, Metabolism Research, Research Tools

Description

Mice homozygous for this Galp (galanin-like peptide) targeted mutation gain less weight than wildtype controls when fed a high fat diet. This mutant mouse strain may be useful in studies of energy homeostasis, nutrition and obesity.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by RT-PCR analysis. Homozygotes are indistinguishable from wildtype when maintained on ad libitum diet. After fasting, male homozygotes consume less food than wildtype controls. When fed a high fat diet homozygotes gain less weight than wildtype controls. This mutant mouse strain may be useful in studies of energy homeostasis, nutrition and obesity.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 2 (contains the translation start site). The construct was electroporated into 129 derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts generated by mating C57BL/6 and B6C3 mice. to C57BL/6 mice to generate the founders, and then crossed to C57BL/6 and C3H mice.

Allele

Symbol: Galp^tm1Stei
Name: targeted mutation 1, Robert A Steiner
MGI accession ID: MGI:3817530
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Galp
Marker name: galanin-like peptide
Marker synonyms: GAL
Chromosome: 7
Strain of origin: 129
Molecular note: Exon 2 was replaced with a neo cassette. The absence of transcript expression was confirmed by RT-PCR analysis.