B6.129P2-Drosha^tm1Litt/J

Stock number: 008592
Research areas: Cell Biology Research

Description

These mice possess loxP sites on either side of exon 9 of the ribonuclease III, nuclear gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 9 is excised in the tissue of interest. Full knockouts of this gene are embryonic lethal before embryonic day 10.5 (E10.5). This strain may be useful in studies of RNA processing.

Detailed description

These mice possess loxP sites on either side of exon 9 of the ribonuclease III, nuclear gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 9 is excised in the tissue of interest. Full knockouts of this gene are embryonic lethal before embryonic day 10.5 (E10.5). This strain may be useful in studies of RNA processing. Homozygotes are viable and fertile.

Development

A targeting vector was used to introduce a loxP-flanked neomycin resistance cassette to intron 8, and an additional loxP site to intron 9 of the gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The neomycin cassette was excised with a C57BL/6 background EIIa-Cre, leaving loxP sites on either side of exon 9. This strain was backcrossed 4 times to C57BL/6 by the donating laboratory.

Allele

Symbol: Drosha^tm1Litt
Name: targeted mutation 1, Dan R Littman
MGI accession ID: MGI:3801076
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Drosha
Marker name: drosha, ribonuclease type III
Chromosome: 15
Strain of origin: 129P2/OlaHsd
Molecular note: A targeting vector was used to introduce a loxP-flanked neomycin resistance cassette to intron 8, and an additional loxP site to intron 9 of the gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The neomycin cassette was excised with a C57BL/6 background EIIa-Cre, leaving loxP sites on either side of exon 9.