B6;129P2-Cxcl14^tm1Litt/J

Stock number: 008590
Research areas: Developmental Biology Research, Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

LacZ was inserted into exon one of the Cxcl14 (chemokine (C-X-C motif) ligand 14) gene. No overt phenotype has as yet been observed. This strain may be useful in studies elucidating the role of this gene. The published literature suggests potential roles in the areas of immunology, development, and diabetes/obesity, among others.

Detailed description

LacZ (expression untested) was inserted into exon one of the gene. No overt phenotype has as yet been observed. This strain may be useful in studies elucidating the role of this gene. The published literature suggests potential roles in the areas of immunology, development, and diabetes/obesity, among others. Homozygotes are viable and fertile.

Development

LacZ cDNA was knocked into exon 1 of the gene immediately after the endogenous start codon ATG. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The line was backcrossed 3 times to C57BL/6.

Allele

Symbol: Cxcl14^tm1Litt
Name: targeted mutation 1, Dan R Littman
MGI accession ID: MGI:3801075
Generation method: Targeted
Marker symbol: Cxcl14
Marker name: chemokine (C-X-C motif) ligand 14
Chromosome: 13
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: LacZ cDNA was knocked into exon 1 of the gene immediately after the endogenous start codon ATG. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The line was backcrossed 3 times to C57BL/6.