B6.129X1-Gpr65^tm1Witt/J

Stock number: 008577
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Homozygous targeted mutant mice have thymocytes and splenocytes that, studied in vitro, are insensitive to pH-dependent cAMP production. A preliminary examination of homozygous animals has not revealed any major abnormalities in the development of the immune system or in its major functions. A green fluorescent protein (GFP) insertion acts as a marker for cells that normally express the targeted gene. This strain may be helpful in elucidating the role of this and other G protein-coupled receptors in the regulation of immune cell responses in acidic microenvironments.

Detailed description

Homozygous targeted mutant mice have thymocytes and splenocytes that, studied in vitro, are insensitive to pH-dependent cAMP production. Despite significant in vitro defects in the acidic pH-induced cAMP production, a preliminary examination of homozygous animals has not revealed any major abnormalities in the development of the immune system or in its major functions. A green fluorescent protein (GFP) insertion acts as a marker for cells that normally express the gene. This strain may be helpful in elucidating the role of this and other G protein-coupled receptors in the regulation of immune cell responses in acidic microenvironments.

Development

A targeting vector was designed to replace exon 2 with IRES-GFP and a PGK-neo cassette flanked by loxP sites. The targeting vector was electroporated into 129X1/SvJ embryonic stem (ES) cells. Clones were injected into C57BL/6 blastocysts. Chimeric animals were backcrossed to C57BL/6 for seven generations.

Allele

Symbol: Gpr65^tm1Witt
Name: targeted mutation 1, Owen N Witte
MGI accession ID: MGI:3530470
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Gpr65
Marker name: G-protein coupled receptor 65
Chromosome: 12
Strain of origin: 129X1/SvJ
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP is expressed in Gpr65-expressing cells.
Molecular note: Most of exon 2 was replaced with an IRES-EGFP and a floxed neo. The absence of transcript expression was confirmed by RT-PCR on spleen and thymus extracts.