Homozygous targeted mutant mice have thymocytes and splenocytes that, studied in vitro, are insensitive to pH-dependent cAMP production. A preliminary examination of homozygous animals has not revealed any major abnormalities in the development of the immune system or in its major functions. A green fluorescent protein (GFP) insertion acts as a marker for cells that normally express the targeted gene. This strain may be helpful in elucidating the role of this and other G protein-coupled receptors in the regulation of immune cell responses in acidic microenvironments.
Owen Witte, University of California, Los Angeles
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Gpr65 | G-protein coupled receptor 65 |
Homozygous targeted mutant mice have thymocytes and splenocytes that, studied in vitro, are insensitive to pH-dependent cAMP production. Despite significant in vitro defects in the acidic pH-induced cAMP production, a preliminary examination of homozygous animals has not revealed any major abnormalities in the development of the immune system or in its major functions. A green fluorescent protein (GFP) insertion acts as a marker for cells that normally express the gene. This strain may be helpful in elucidating the role of this and other G protein-coupled receptors in the regulation of immune cell responses in acidic microenvironments.
A targeting vector was designed to replace exon 2 with IRES-GFP and a PGK-neo cassette flanked by loxP sites. The targeting vector was electroporated into 129X1/SvJ embryonic stem (ES) cells. Clones were injected into C57BL/6 blastocysts. Chimeric animals were backcrossed to C57BL/6 for seven generations.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP is expressed in Gpr65-expressing cells. |
Allele Name | targeted mutation 1, Owen N Witte |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | TDAG8- |
Gene Symbol and Name | Gpr65, G-protein coupled receptor 65 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is expressed in Gpr65-expressing cells. |
Strain of Origin | 129X1/SvJ |
Chromosome | 12 |
Molecular Note | Most of exon 2 was replaced with an IRES-EGFP and a floxed neo. The absence of transcript expression was confirmed by RT-PCR on spleen and thymus extracts. |
Mutations Made By | Owen Witte, University of California, Los Angeles |
When maintained as a live colony, heterozyogotes or homozygotes may be bred.
When using the B6.129X1-Gpr65tm1Witt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008577 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gpr65<tm1Witt> |
Frozen Mouse Embryo | B6.129X1-Gpr65<tm1Witt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Gpr65<tm1Witt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Gpr65<tm1Witt>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Gpr65<tm1Witt>/J Frozen Embryo | $3373.50 |
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