B6.129S6(FVB)-Kdm5a^tm1.1Kael/J

Stock number: 008571
Research areas: Cancer Research, Cell Biology Research

Description

Cells from these Kdm5a (lysine (K)-specific demethylase 5A; also called RBP2, retinoblastoma binding protein 2) targeted mutation mice display enhanced transcription of certain cytokine genes which are associated with increased histone H3 lysine 4 (H3K4) trimethylation. Homozygous mice on this C57BL/6 background die right after birth while heterozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain may be useful in mechanistic studies of transcriptional regulation.

Detailed description

Cells from these targeted mutation mice display enhanced transcription of certain cytokine genes which are associated with increased histone H3 lysine 4 (H3K4) trimethylation. Homozygous mice on this C57BL/6 background die right after birth while heterozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice on a 129-C57BL/6-FVB mixed background are viable, but 20-30% smaller in size, and their hind legs tend to tremble in a tail suspension position. This strain may be useful in mechanistic studies of transcriptional regulation.

Development

A conditional targeting vector was generated by flanking exons 5 and 6 with a 5' loxP site and a 3' Frt-PGK-neomycin-Frt-LoxP expression cassette. After electroporation of 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells, correctly targeted ES clones were injected into C57BL/6 blastocysts, and chimeric mice were bred into C57BL/6. Germline transmitting mice were bred with EIIa-Cre transgenic mice in FVB background to generate the null allele. The resulting heterozygous mice were then backcrossed to C57BL/6 for 7 generations.

Allele

Symbol: Kdm5a^tm1.1Kael
Name: targeted mutation 1.1, William G Kaelin
MGI accession ID: MGI:3798768
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Kdm5a
Marker name: lysine (K)-specific demethylase 5A
Chromosome: 6
Strain of origin: 129S6/SvEvTac
Molecular note: A targeting vector was generated by flanking exons 5 and 6 with a 5' loxP site and a 3' Frt-PGK-neomycin-Frt-LoxP expression cassette. Germline transmitting mice were bred sequentially with CAGG-Flpe mice to remove the neo cassette, and then to EIIa-Cre transgenic mice in FVB background to generate the null allele.