Cells from these Kdm5a (lysine (K)-specific demethylase 5A; also called RBP2, retinoblastoma binding protein 2) targeted mutation mice display enhanced transcription of certain cytokine genes which are associated with increased histone H3 lysine 4 (H3K4) trimethylation. Homozygous mice on this C57BL/6 background die right after birth while heterozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain may be useful in mechanistic studies of transcriptional regulation.
William G. Kaelin, Dana Farber Cancer Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Kdm5a | lysine (K)-specific demethylase 5A |
Cells from these targeted mutation mice display enhanced transcription of certain cytokine genes which are associated with increased histone H3 lysine 4 (H3K4) trimethylation. Homozygous mice on this C57BL/6 background die right after birth while heterozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice on a 129-C57BL/6-FVB mixed background are viable, but 20-30% smaller in size, and their hind legs tend to tremble in a tail suspension position. This strain may be useful in mechanistic studies of transcriptional regulation.
A conditional targeting vector was generated by flanking exons 5 and 6 with a 5' loxP site and a 3' Frt-PGK-neomycin-Frt-LoxP expression cassette. After electroporation of 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells, correctly targeted ES clones were injected into C57BL/6 blastocysts, and chimeric mice were bred into C57BL/6. Germline transmitting mice were bred with EIIa-Cre transgenic mice in FVB background to generate the null allele. The resulting heterozygous mice were then backcrossed to C57BL/6 for 7 generations.
Allele Name | targeted mutation 1.1, William G Kaelin |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Kdm5a, lysine (K)-specific demethylase 5A |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 6 |
Molecular Note | A targeting vector was generated by flanking exons 5 and 6 with a 5' loxP site and a 3' Frt-PGK-neomycin-Frt-LoxP expression cassette. Germline transmitting mice were bred sequentially with CAGG-Flpe mice to remove the neo cassette, and then to EIIa-Cre transgenic mice in FVB background to generate the null allele. |
Mutations Made By | William Kaelin, Dana Farber Cancer Institute |
When maintained as a live colony, heterozygotes may be bred. Homozygotes die within an hour after birth.
When using the B6.129S6(FVB)-Kdm5atm1.1Kael/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008571 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Rbp2<tm1.1Kael> |
Frozen Mouse Embryo | B6.129S6(FVB)-Kdm5a<tm1.1Kael>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6(FVB)-Kdm5a<tm1.1Kael>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6(FVB)-Kdm5a<tm1.1Kael>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S6(FVB)-Kdm5a<tm1.1Kael>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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