Mice harboring the hCD2-iCre transgene have the human CD2 promoter and locus control region (LCR) directing expression of an optimized variant of Cre recombinase (iCre) to T cells and B cells (all B cell and T cell progenitors) and mice may be useful for generating conditional mutations in T cells and B cells. Because of the integration site of the transgene, the hCD2-iCre transgene is linked to the agouti coat color genes.
Dimitris Kioussis, MRC Natl Inst for Med Research
Genetic Background | Generation |
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N17+pN2F1
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
Mice hemizygous for this hCD2-iCre transgene are viable and fertile, with the human CD2 promoter and locus control region (LCR) directing expression of an optimized variant of Cre recombinase (iCre) to T cells and B cells (all committed B cell and T cell progenitors). Using crosses to a reporter strain, variegated germ line (testis) and a small monocyte-enriched population expression is also reported. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These hCD2-iCre transgenic mice may be useful for generating conditional mutations in T cells and B cells.
Of note, this hCD2-iCre strain (Stock No. 008520) allows reliable deletion/gene targeting to be focused to T cells and B cells, whereas the Vav-iCre strain (Stock No. 008610) allows targeting throughout the entire hematopoietic compartment. hCD2-cre mice (Stock No. 027406) allow efficient deletion of genes in peripheral CD4+ and CD8+ T cells after positive selection in the thymus.
IMPORTANT NOTE: The donating investigator initially reported that while backcrossing these hCD2-iCre transgenic mice to C57BL/6 mice, transgenic mice always exhibited agouti coat color. This linkage suggests that the hCD2-iCre transgene integrated near agouti coat color genes. Due to a recombination event in the JAX colony (May 2015) the coat color is now black.
The hCD2-iCre transgene was designed with the human CD2 promoter, an optimized variant of Cre recombinase (iCre; improved with mammalian codon usage, removed putative cryptic splice sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), an SV40 polyA signal, and the human CD2 locus control region (LCR). The transgene was microinjected into the pronuclei of fertilized oocytes from (CBA/Ca x C57BL/10) Fl mice. Founder mice were established (line ".4") and used to generate mutant mice. The resulting hCD2-iCre transgenic mice were subsequently bred to wildtype C57BL/6 mice for more than 15 generations prior to arrival at The Jackson Laboratory Repository. Upon arrival, the hCD2-iCre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | expressed in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches and testes. |
Allele Name | transgene insertion 4, Dimitris Kioussis |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | CD2-Cre; hCD2-Cre; hCD2-iCre; Tg(CD2-cre)1Kio; Tg(CD2-cre)4Kio |
Gene Symbol and Name | Tg(CD2-icre)4Kio, transgene insertion 4, Dimitris Kioussis |
Gene Synonym(s) | |
Promoter | CD2, CD2 molecule, human |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | expressed in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches and testes. |
Strain of Origin | (CBA/Ca x C57BL/10)F2 |
Chromosome | UN |
Molecular Note | This transgene expresses Cre recombinase under the control of a human CD2 promoter. The promoter was shown to be active in lymph nodes, thymus, spleen, Peyer's patches, intestinal cryptopatches and testes. |
Mutations Made By | Dimitris Kioussis, MRC Natl Inst for Med Research |
Because of the integration site of the transgene, hCD2-iCre transgenic mice initially had an agouti coat color. Due to a recombination event in the JAX colony (May 2015) the coat color is now black. When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57BL/6J. The donating investigator reports that homozygous mice are not viable.
When using the hCD2-iCre mouse strain in a publication, please cite the originating article(s) and include JAX stock #008520 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non Carrier for Tg(CD2-icre)4Kio |
Frozen Mouse Embryo | B6.Cg-Tg(CD2-icre)4Kio/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(CD2-icre)4Kio/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(CD2-icre)4Kio/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(CD2-icre)4Kio/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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