B6;129X1-Wnt7b^tm2Amc/J

Stock number: 008467
Research areas: Cell Biology Research, Developmental Biology Research, Internal/Organ Research, Research Tools

Description

These Wnt7b^c3 mice harbor loxP sites flanking exon 3 of the Wnt7b (wingless-related MMTV integration site 7B) locus and may be useful in studying the role of Wnt7b (and other Wnt family members) in development and canonical Wnt signaling cascades, including lung differentiation and growth.

Detailed description

Mice homozygous for the Wnt7b^c3 allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Unlike other Wnt7b mutant alleles, this Wnt7b^c3 conditional allele is not affected by alternative exon 1 splicing. These Wnt7b^c3 mice may be useful in generating conditional mutations for studying the role of Wnt7b (and other Wnt family members) in development and canonical Wnt signaling cascades, including lung differentiation and growth. In addition, these mice may also be useful in conjunction with other Wnt7 mutant strains including Wnt7b knockout mice (Stock No. 004693) and Wnt7a mutant mice (Stock No. 004715).

When bred to a strain expressing Cre recombinase in epiblast cells (see Stock No. 008454, 004783 for example), this mutant mouse strain may be useful in studies of embronic lung growth.

When bred to a strain expressing Cre recombinase in the mesonephric duct and its' developmental derivatives (see Stock No. 004692 for example), this mutant mouse strain may be useful in studies of kidney development.

Development

A targeting vector was designed to insert loxP sites on both sides of exon 3 of the targeted gene, as well as insert an frt-flanked PGK-neo cassette downstream of exon 3. The donating investigator reports that this construct was microinjected into 129X1/SvJ-derived AV3 embryonic stem (ES) cells, correctly targeted ES cells were injected into recipient blastocysts, and chimeric mice were bred with C57BL/6 mice. The resulting Wnt7b^c3 mutant mice were next backcrossed to C57BL/6 mice for a few generations (and confirmed to still harbor the frt-flanked PGK-neo cassette downstream of exon 3) prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony. It was then determined that these mice are on a mixed C57BL/6J and 129X1/SvJ genetic background.

Allele

Symbol: Wnt7b^tm2Amc
Name: targeted mutation 2, Andrew P McMahon
MGI accession ID: MGI:3526432
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Wnt7b
Marker name: wingless-type MMTV integration site family, member 7B
Chromosome: 15
Strain of origin: 129X1/SvJ
Molecular note: Exon 3 was floxed and an frt flanked PGK-neo cassette was inserted downstream of exon 3.