These Wnt7bc3 mice harbor loxP sites flanking exon 3 of the Wnt7b (wingless-related MMTV integration site 7B) locus and may be useful in studying the role of Wnt7b (and other Wnt family members) in development and canonical Wnt signaling cascades, including lung differentiation and growth.
Andrew P McMahon, University of Southern California
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Wnt7b | wingless-type MMTV integration site family, member 7B |
Mice homozygous for the Wnt7bc3 allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Unlike other Wnt7b mutant alleles, this Wnt7bc3 conditional allele is not affected by alternative exon 1 splicing. These Wnt7bc3 mice may be useful in generating conditional mutations for studying the role of Wnt7b (and other Wnt family members) in development and canonical Wnt signaling cascades, including lung differentiation and growth. In addition, these mice may also be useful in conjunction with other Wnt7 mutant strains including Wnt7b knockout mice (Stock No. 004693) and Wnt7a mutant mice (Stock No. 004715).
When bred to a strain expressing Cre recombinase in epiblast cells (see Stock No. 008454, 004783 for example), this mutant mouse strain may be useful in studies of embronic lung growth.
When bred to a strain expressing Cre recombinase in the mesonephric duct and its' developmental derivatives (see Stock No. 004692 for example), this mutant mouse strain may be useful in studies of kidney development.
A targeting vector was designed to insert loxP sites on both sides of exon 3 of the targeted gene, as well as insert an frt-flanked PGK-neo cassette downstream of exon 3. The donating investigator reports that this construct was microinjected into 129X1/SvJ-derived AV3 embryonic stem (ES) cells, correctly targeted ES cells were injected into recipient blastocysts, and chimeric mice were bred with C57BL/6 mice. The resulting Wnt7bc3 mutant mice were next backcrossed to C57BL/6 mice for a few generations (and confirmed to still harbor the frt-flanked PGK-neo cassette downstream of exon 3) prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony. It was then determined that these mice are on a mixed C57BL/6J and 129X1/SvJ genetic background.
Allele Name | targeted mutation 2, Andrew P McMahon |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Wnt7bC3 |
Gene Symbol and Name | Wnt7b, wingless-type MMTV integration site family, member 7B |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 15 |
Molecular Note | Exon 3 was floxed and an frt flanked PGK-neo cassette was inserted downstream of exon 3. |
Mutations Made By | Andrew McMahon, University of Southern California |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129X1-Wnt7btm2Amc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008467 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Wnt7b<tm2Amc> |
Frozen Mouse Embryo | B6;129X1-Wnt7b<tm2Amc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129X1-Wnt7b<tm2Amc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129X1-Wnt7b<tm2Amc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129X1-Wnt7b<tm2Amc>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.