This strain enables temporal control of floxed gene expression by tamoxifen induction in vivo.
Dr. Tyler Jacks, Massachusetts Institute of Technology
Genetic Background | Generation |
---|---|
N9+N2F4
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing, Inducible) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
A conditional Cre-ERT2 (Cre recombinase - estrogen receptor T2) cassette was introduced to the gene. The ERT2 moiety retains the Cre recombinase in the cytoplasm until tamoxifen administration releases this inhibition, thus permitting the recombination of genomic loxP sites. Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. This strain enables temporal control of floxed gene expression in vivo and is reportedly more sensitive to tamoxifen than Stock No. 004847. Homozygotes are viable and fertile.
A conditional Cre-ERT2 (estrogen receptor T2) cassette was inserted to intron 1 of the gene. The mutation was created in 129S4/SvJae-derived J1 embryonic stem (ES) cells and backcrossed 8 times to C57BL/6 by the donating laboratory.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
---|---|
Site of Expression | Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. |
Allele Name | targeted mutation 1, Tyler Jacks |
---|---|
Allele Type | Targeted (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Cre-ERT2; Gt(ROSA)26Sortm1(cre/ESR1)Tyj; R26-Cre-ERT2; Rosa26CreER |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. |
Strain of Origin | 129S4/SvJae |
Chromosome | 6 |
Molecular Note | A cassette containing Cre-ERT2 was inserted into the locus. |
Mutations Made By | Dr. Tyler Jacks, Massachusetts Institute of Technology |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the R26-CreERT2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #008463 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Gt(ROSA)26Sor<tm1(cre/ESR1)Tyj> |
Frozen Mouse Embryo | B6.129-Gt(ROSA)26Sor<tm1(cre/ERT2)Tyj>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Gt(ROSA)26Sor<tm1(cre/ERT2)Tyj>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Gt(ROSA)26Sor<tm1(cre/ERT2)Tyj>/J | $3373.50 |
Frozen Mouse Embryo | B6.129-Gt(ROSA)26Sor<tm1(cre/ERT2)Tyj>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.