B6.129-Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}/J

Stock number: 008463
Product name: R26-CreERT2
Research areas: Research Tools

Description

This strain enables temporal control of floxed gene expression by tamoxifen induction in vivo.

Detailed description

A conditional Cre-ER^T2 (Cre recombinase - estrogen receptor T2) cassette was introduced to the gene. The ER^T2 moiety retains the Cre recombinase in the cytoplasm until tamoxifen administration releases this inhibition, thus permitting the recombination of genomic loxP sites. Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. This strain enables temporal control of floxed gene expression in vivo and is reportedly more sensitive to tamoxifen than Stock No. 004847. Homozygotes are viable and fertile.

Development

A conditional Cre-ER^T2 (estrogen receptor T2) cassette was inserted to intron 1 of the gene. The mutation was created in 129S4/SvJae-derived J1 embryonic stem (ES) cells and backcrossed 8 times to C57BL/6 by the donating laboratory.

Allele

Symbol: Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}
Name: targeted mutation 1, Tyler Jacks
MGI accession ID: MGI:3699244
Generation method: Targeted
Attributes: Recombinase-expressing; Inducible
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: 129S4/SvJae
Expressed genes: cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain.
Molecular note: A cassette containing Cre-ERT2 was inserted into the locus.