p53flox mice have loxP sites flanking exons 2-10 of the transformation related protein 53 gene. Exposure to Cre recombinase removes the floxed sequence - creating a null allele. These p53flox mice may be useful in studying cancer, tumor suppression, cell-cycle-arrest, apoptosis and cellular stress signalling.
Dr. Anton Berns, University of Amsterdam
Exons 2-10 are flanked by loxP sites in this conditional targeted mutant strain. Mice homozygous for the floxed allele do not show any increase in disease incidence for at least a year. When bred to mice with a Cre recombinase gene under the control of a promoter of interest, expression is deleted in the tissue of interest.
For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of medulloblastoma formation.
When crossed to a strain expressing Cre recombinase in the central nervous system (see Stock No. 004600), this mutant mouse strain may be useful in studies of astrocytoma formation.
When crossed to a strain expressing Cre recombinase in virgin and lactating mammary glands (see Stock No. 003553), this mutant mouse strain may be useful in studies of mammary gland tumors.
When crossed to a strain expressing a doxycyclin-inducible Cre recombinase in the osteoblast lineage (see Stock No. 006361), this mutant mouse strain may be useful in studies of osteosarcomas.
When crossed to a strain expressing Cre recombinase in the epithelial cells of the developing kidney and genitourinary tract (see Stock No. 012237), this mutant mouse strain may be useful in studies of endometrial carcinoma.
A targeting vector was used to introduce flanking loxP sites to introns 1 and 10 of the gene. An IB10/E14IB10 129P2/OlaHsd-derived embryonic stem cell line was used to create the mutation. This line has been backcrossed to C57BL/6 for eight generations by the donating laboratory of Dr. Tyler Jacks (see SNP note below).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Anton Berns|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||p53Co; p53F; p53F2-10; p53F2-10F; p53fl; p53flox; p53L; p53lox; p53LoxP; p53Fl; Tp53flx; Trp53f; Trp53F2-10; Trp53F2-F10; Trp53Fl; Trp53loxP|
|Gene Symbol and Name||Trp53, transformation related protein 53|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Insertion of loxP sites flanking exons 2 through 10. No effect on the normal function of the gene.|
|Mutations Made By|| |
Dr. Anton Berns, University of Amsterdam
When maintained as a live colony, heterozygotes may be bred. Homozygotes have a somewhat reduced fertility, but may be bred.
When using the p53LoxP mouse strain in a publication, please cite the originating article(s) and include JAX stock #008462 in your Materials and Methods section.
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