STOCK Mirc1^tm1.2Tyj/J

Stock number: 008459
Research areas: Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

The miR-17~92 (microRNA17-92) cluster, overexpressed in human cancers, is deleted in this targeted mutant strain. Mice homozygous for this null allele (miR-17~92^delta) die perinatally, displaying cardiac defects and hypoplastic lungs. In fetal liver reconstitution experiments, a defect in B-cell development (partial block at the pro-B to pre-B transition) is observed. This strain may be useful in studying the link between the region's oncogenic properties and its functions during B cell lymphopoiesis and lung development.

Detailed description

The miR-17~92 (Mir17, Mir18, Mir19a, Mir20a, Mir19b-1, Mir92-1) cluster, overexpressed in human cancers, is deleted in this targeted mutant strain. Heterozygous mice are viable and fertile. Mice homozygous for this null allele (miR-17~92^delta) die perinatally, displaying cardiac defects and hypoplastic lungs. In fetal liver reconstitution experiments, a defect in B-cell development (partial block at the pro-B to pre-B transition) is observed. This strain may be useful in studying the link between the region's oncogenic properties and its functions during B cell lymphopoiesis and lung development.

Development

loxP sites were introduced 5' of the Mirn17 gene and 3' of the Mirn92-1 gene. The floxed region was followed by an Frt-flanked neomycin cassette. The targeting vector was introduced to V6.5 (C57BL/6 x 129S4/SvJae)F1-derived embryonic stem (ES) cells. The neomycin cassette was removed through Flp-mediated excision via a C57BL/6 background strain. Crosses with a C57BL/6 background Cre deleter strain (human ACTB beta actin promoter) excised the floxed genes. This strain was maintained on a mixed C57BL/6 and 129S4 background by the donating laboratory.

Allele

Symbol: Mirc1^tm1.2Tyj
Name: targeted mutation 1.2, Tyler Jacks
MGI accession ID: MGI:3790968
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Mirc1
Marker name: microRNA cluster 1, including Mir17 through Mir92-1
Chromosome: 14
Strain of origin: (C57BL/6 x 129S4/SvJae)F1
Molecular note: Crossing Mirc1^tm1.1Tyj mice to a Cre deleter strain (human ACTB beta actin promoter) excised the floxed cluster, including Mir17, Mir18a, Mir19a, Mir20a, Mir19b-1, and Mir92-1. This results in a null allele.