These mice, carrying a targeted mutation of the fibronectin 1 gene (Fn1), have been used in a search for cardiac development modifier genes demonstrating phenotypic differences on C57BL/6J and 129S4/SvJae genetic backgrounds (Astrof 2007). On the C57BL/6J genetic background, cardiac development progresses further than that of 129S4 background mice, and results in a centrally-positioned looped heart. This strain may be helpful in studies of cardiac development.
Dr. Richard Hynes, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fn1 | fibronectin 1 |
These mice carry a targeted mutation in the fibronectin 1 gene identical to that found in Stock No. 002270.
This line, backcrossed more than 13 times to C57BL/6J in the donating investigator's colony, has been used in a search for cardiac development modifier genes demonstrating phenotypic differences on C57BL/6J and 129S4/SvJae genetic backgrounds (Astrof, 2007).
In the majority of 129S4 background homozygous targeted mutant embryos, heart progenitors remain at their anterior bilateral positions and fail to fuse at the midline to form a heart tube. However, on the C57BL/6J genetic background, cardiac development progresses further and results in a centrally-positioned looped heart.
Linkage analysis led to the identification of a 1-Mbp interval on mouse Chromosome 4 containing 21 genes, including five that are differentially expressed between C57BL/6J and 129S4/SvJae.
This strain may be helpful in studies of cardiac development.
A PGK-neomycin resistance cassette replaced 0.8 kb of the targeted gene including the translation initiation site and part of the signal sequence. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells. The mice were bred to 129S4/SvJae then backcrossed to C57BL/6J in the donating investigator's colony for at least 13 generations. The strain was sperm-cryopreserved and will typically be recovered using C57BL/6J females maintained at The Jackson Laboratory.
Allele Name | targeted mutation 1, Richard Hynes |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | FN.null; Fn-; FNnull |
Gene Symbol and Name | Fn1, fibronectin 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 1 |
Molecular Note | A PGK-neomycin resistance cassette replaced 0.8 kb of the gene including the translation initiation site and part of the signaling sequence. Plasma concentrations of fibronectin in heterozygotes were one-half those of wild-type littermates. The encoded protein was not detectable in immunoprecipitations from cultures of homozygous mutant E7.5 embryos. |
Mutations Made By | Dr. Richard Hynes, Massachusetts Institute of Technology |
When maintained as a live colony, heterozygotes may be bred. Homozygotes show embryonic lethality.
When using the B6.129S-Fn1tm1Hyn/2J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008445 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Fn1<tm1Hyn> |
Frozen Mouse Embryo | B6.129S-Fn1<tm1Hyn>/2J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Fn1<tm1Hyn>/2J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Fn1<tm1Hyn>/2J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S-Fn1<tm1Hyn>/2J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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