B6.Cg-Tg(Tcra,Tcrb)HRCAll/J

Stock number: 008428
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

HRC transgenic mice express a transgene encoding a T cell receptor (TCR) αβ heterodimer specific for histone H4 (86-92) peptide VVYALKR presented by the H-2Kb class I MHC molecule. The TCRαβ transgene is expressed on >90% of mature T cells. Mice that are hemizygous for the transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. This mouse strain may be useful for studies of immune regulation, cancer, and autoimmunity.

Detailed description

HRC transgenic mice express a transgene encoding a T cell receptor (TCR) αβ heterodimer specific for histone H4 (86-92) peptide VVYALKR presented by the H-2K^b class I MHC molecule. The TCRαβ transgene is expressed on >90% of mature T cells. Mice that are hemizygous for the transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. This mouse strain may be useful for studies of immune regulation, cancer, and autoimmunity.

Development

Rearranged V α2-SGTGGYKV Tcra and V α8.3-SGTGGSAETL Tcrb chains recognizing histone H4 (86-92) peptide VVYALKR were identified in a population of CD8⁺ T cells infiltrating mouse prostate tumors. These were cloned into separate targeting constructs. Linearized sequences were co-injected into (CBAxC57BL/6)F2 embryos and resultant mice were backcrossed more than ten times to C57BL/6 by the donating laboratory. This line gives "complete" expression of the transgenic TCR αβ heterodimer on >90% of CD8α⁺ cells.

Allele

Symbol: Tg(Tcra,Tcrb)HRCAll
Name: transgene insertion HRC, James P Allison
MGI accession ID: MGI:3773741
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Tcra,Tcrb)HRCAll
Marker name: transgene insertion HRC, James P Allison
Chromosome: UN
Strain of origin: either: C57BL/6 or (CBA x C57BL/6)F2
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory; Tcrb,T cell receptor beta chain,mouse, laboratory
Molecular note: Rearranged Valpha2-SGTGGYKV TCRalpha and Vbeta8.3-SGTGGSAETL TCRbeta chains were cloned from a population of CD8⁺ T cells that recognizes a 17-mer peptide from the C-terminus of Histone 4 family members presented on H2-K^b. The recombined gene segments were placed in a TCR expression vector and injected into blastocytes. This founder line is characterized by "complete" expression of the transgenic TCR with 98% of CD8⁺ T cells expressing the transgenic TCR.