These compound mutant mice carry targeted mutations of the Stx1a (syntaxin 1A (brain)) and Stx1b (syntaxin 1B) genes. Double homozygous mice die prenatally, show a 40% reduction in syntaxin binding protein (Munc-18) levels and have small Purkinje cells in cerebellum.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
|Allele Type||Gene Symbol||Gene Name|
|Targeted||Stx1a||syntaxin 1A (brain)|
|Allele Type||Gene Symbol||Gene Name|
These compound mutant mice carry targeted mutations of the Stx1a (syntaxin 1A (brain)) and Stx1b (syntaxin 1B) genes. Stx1a mutant (XRA) homozygous mice do not show any abnormality of their own, but in combination with the Stx1b hypomorph mutation (XTB) (see Stock No. 008138), XRA worsens the XRB phenotype. Double homozygous mice die prenatally, show a 40% reduction in syntaxin binding protein (Munc-18) levels and have small Purkinje cells in cerebellum.
This compound mutant strain was created by crossing "XRA" (Stx1a mutant) mice with "XRB" (Stx1b mutant) mice.
To create the XRA mice (see Stock No. 008137), a targeting vector was used to introduce a Flp-flanked neomycin resistance cassette to intron 1 and a loxP-flanked green fluorescent protein (GFP) segment to exon 2 of the gene. The GFP segment was deleted from targeted animals through crosses with a Cre strain. The background of this mouse has not been indicated. Upon arrival, mice were bred to C57BL/6J mice for at least one generation to establish the colony.
The XRB mice were derived from XTB mice (see Stock No. 008138). XTB mice were created using a vector designed to introduce two modified copies of exon 7 to the targeted gene. The first modified exon 7 carries a partial cDNA fragment of syntaxin with a C-terminal GFP and a stop codon. This mutation is followed by neomycin resistance cassette placed in intron 7 and flanked by flp sites. The first exon 7 insertion and the neomycin cassette are flanked by a pair of Frt sites. A second modified exon 7 bearing L165A and E166A mutations follows the neomycin insertion. The background of this mouse has not been indicated. Upon arrival, mice were bred to C57BL/6J mice for at least one generation to establish the colony. To create the XRB line, crosses with a mixed C57BL/6 and 129 background Cre strain excised the floxed region (incorporating the exon 7 GFP knock-in and neomycin cassette).
The two independent XRA and XRB lines were intercrossed to create this compound mutant line.
|Allele Name||targeted mutation 1.1, Thomas C Sudhof|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Stx1a, syntaxin 1A (brain)|
|Gene Synonym(s)||HPC-1; P35-1; STX1; SYN1A|
|Strain of Origin||Not Specified|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
|Allele Name||targeted mutation 1.1, Thomas C Sudhof|
|Allele Type||Targeted (Null/Knockout, Reporter)|
|Allele Synonym(s)||XRB; syntaxin-1BOpen|
|Gene Symbol and Name||Stx1b, syntaxin 1B|
|Gene Synonym(s)||GEFSP9; STX1B1; STX1B2; Stx1b2; Stx1b2; Stx2; syntaxin 1B2|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl<+>|
When maintained as a live colony animals homozygous for the Stx1a mutation and heterozygous for the Stx1b2 mutation may be intercrossed. Animals homozygous for the Stx1b2 mutation die around postnatal day 30 due to seizures.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
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