Overview

Also Known As:NBR

These conditional targeted mutation mice carry loxP sites on either side of the first unique coding exon of the neurexin beta promoter isoforms of Nrxn1 (neurexin I), Nrxn2 (neurexin II), and Nrxn3 (neurexin III). These exons correspond to exon 18 of Nrxn1 (neurexin I), exon 17 of Nrxn2 (neurexin II), and exon 17 of Nrxn3 (neurexin III). When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the beta isoforms of the gene(s). This strain may be useful in studies of neuronal synaptic vesicle exocytosis.

Note: The original curations for this strain indicated that exon 1 of the Nrxn1, Nrxn2, and Nrxn3 genes were modified. Subsequent published information (Anderson, 2015) clarified that it was the first exon of the beta promoter isoforms that were targeted. (September, 2019)

Donating Investigator

Dr. Thomas C. Sudhof, Stanford University School of Medicine

Genetic overview

Genetic Background	Generation

Nrxn3^tm2Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Nrxn3	neurexin III

Nrxn1^tm2Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Nrxn1	neurexin I

Nrxn2^tm2Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Nrxn2	neurexin II

View Genetics

Research Applications

Neurobiology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

These conditional targeted mutation mice carry loxP sites on either side of the first unique coding exon of the neurexin beta promoter isoforms of Nrxn1 (neurexin I), Nrxn2 (neurexin II), and Nrxn3 (neurexin III). These exons correspond to exon 18 of Nrxn1 (neurexin I), exon 17 of Nrxn2 (neurexin II), and exon 17 of Nrxn3 (neurexin III). These exons have been further modified to include green fluorescent protein (GFP) (neurexins 1 and 3), or hemagglutinin (HA) protein (neurexin 2). The GFP and HA inserts don't affect the expression of the beta neurexin genes, but it is very difficult to detect signals in the tissue by immunohistochemistry or direct fluorescence. The single, double and triple homozygous floxed mutants are fully viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the beta isoforms of the gene(s). This strain may be useful in studies of neuronal synaptic vesicle exocytosis.

Homozygous deletion of the beta neurexin 3 floxed segment mediated through Cre excision generates pups with normal viability and fertility; triple knockout mice are viable, but are infertile and smaller in size (~70% of wild-type in body weight).

Note: The original curations for this strain indicated that exon 1 of the Nrxn1, Nrxn2, and Nrxn3 genes were modified. Subsequent published information (Anderson, 2015) clarified that it was the first exon of the beta promoter isoforms that were targeted. (September, 2019).

Development

Each of the three targeted genes were modified by the insertion of a loxP site in the 5' UTR, and an Frt-flanked neomycin cassette with a 3' loxP site in the first unique coding exon of the neurexin beta promoter isoforms of Nrxn1 (neurexin I), Nrxn2 (neurexin II), and Nrxn3 (neurexin III). These exons correspond to exon 18 of Nrxn1 (neurexin I), exon 17 of Nrxn2 (neurexin II), and exon 17 of Nrxn3 (neurexin III). These exons have been further modified to include green fluorescent protein (GFP) (neurexins 1 and 3), or hemagglutinin (HA) protein (neurexin 2).

The GFP and HA inserts don't affect the expression of beta neurexin genes, but it is very difficult to detect GFP and HA signals in the tissue by immunohistochemistry or direct fluorescence. The genes were independently targeted in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Resultant germline mutant mice were crossed with a transgenic flipase (FLP) strain on a mixed C57BL/6 and 129 background to remove the neomycin cassettes. The three mutations were intercrossed and backcrossed three or more times to C57BL/6.

Note: The original curations for this strain indicated that exon 1 of the Nrxn1, Nrxn2, and Nrxn3 genes were modified. Subsequent published information (Anderson, 2015) clarified that it was the first exon of the beta promoter isoforms that were targeted. (September, 2019).

Selected References

Anderson GR; Aoto J; Tabuchi K; Foldy C; Covy J; Yee AX; Wu D; Lee SJ; Chen L; Malenka RC; Sudhof TC. 2015. beta-Neurexins Control Neural Circuits by Regulating Synaptic Endocannabinoid Signaling. Cell 162(3):593-606PubMed: 26213384 MGI: J:224592

View All References

Genetics

Nrxn3^tm2Sud

Allele Symbol: Nrxn3^tm2Sud

Allele Name	targeted mutation 2, Thomas C Sudhof
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Nrxn3beta cKO
Gene Symbol and Name	Nrxn3, neurexin III
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	12
Molecular Note	A loxP site was inserted into the 5' end of a modified exon 17, which contains an EGFP reporter gene followed by a FRT-flanked neomycin resistance cassette and a loxP site. Flp-mediated recombination removed the selection cassette and left the modified exon 17 floxed. The GFP insert does not affect the expression of neurexin 3 gene, but it is very difficult to detect GFP signals in the tissue by immunohistochemistry or direct fluorescence.

Nrxn1^tm2Sud

Allele Symbol: Nrxn1^tm2Sud

Allele Name	targeted mutation 2, Thomas C Sudhof
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Nrxn1beta cKO
Gene Symbol and Name	Nrxn1, neurexin I
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	17
Molecular Note	A loxP site was inserted into the 5' end of a modified exon 18, which contains an EGFP reporter gene followed by FRT-flanked neomycin resistance cassette and a loxP site. Flp-mediated recombination removed the selection cassette and left the modified exon 18 floxed. The GFP insert does not affect the expression of neurexin 1 gene, but it is very difficult to detect GFP signals in the tissue by immunohistochemistry or direct fluorescence.

Nrxn2^tm2Sud

Allele Symbol: Nrxn2^tm2Sud

Allele Name	targeted mutation 2, Thomas C Sudhof
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Nrxn2beta cKO
Gene Symbol and Name	Nrxn2, neurexin II
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	19
Molecular Note	A loxP site was inserted into the 5' end of a modified exon 17, which contains an hemagglutinin (HA) reporter tag followed by a FRT-flanked neomycin resistance cassette and a loxP site. Flp-mediated recombination removed the selection cassette and left the modified exon 17 floxed. The HA insert does not affect the expression of neurexin 2 gene, but it is very difficult to detect GFP signals in the tissue by immunohistochemistry.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Neurotransmitter Receptor and Synaptic Vesicle Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Nrxn1^tm2Sud/Nrxn1^tm2Sud Nrxn2^tm2Sud/Nrxn2^tm2Sud Nrxn3^tm2Sud/Nrxn3^tm2Sud
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

no abnormal phenotype detected
- Normal - mice are fully viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities

References

Anderson GR; Aoto J; Tabuchi K; Foldy C; Covy J; Yee AX; Wu D; Lee SJ; Chen L; Malenka RC; Sudhof TC. 2015. beta-Neurexins Control Neural Circuits by Regulating Synaptic Endocannabinoid Signaling. Cell 162(3):593-606PubMed: 26213384 MGI: J:224592

Additional References

Wu X; Morishita WK; Riley AM; Hale WD; Sudhof TC; Malenka RC. 2019. Neuroligin-1 Signaling Controls LTP and NMDA Receptors by Distinct Molecular Pathways. Neuron 102(3):621-635.e3PubMed: 30871858 MGI: J:276060

Additional - Nrxn1^tm2Sud related

Additional - Nrxn2^tm2Sud related

Additional - Nrxn3^tm2Sud related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Nrxn2
- Standard PCR:Nrxn1
- Standard PCR:Nrxn3
- Genotyping resources and troubleshooting
Breeding Considerations

When maintained as a live colony, triple homozygous floxed mice may be intercrossed.
- Additional Breeding and Husbandry Support
Citation
When using the NBR mouse strain in a publication, please cite the originating article(s) and include JAX stock #008416 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Inbred, 1 pair minimum will be suppliedHomozygous for Nrxn3<tm2Sud>,Homozygous for Nrxn1<tm2Sud>, Homozygous for Nrxn2<tm2Sud>

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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General Terms and Conditions

Questions about Terms of Use

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Also Known As:NBR

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Selected References

Nrxn3tm2Sud

Allele Symbol: Nrxn3tm2Sud

Nrxn1tm2Sud

Allele Symbol: Nrxn1tm2Sud

Nrxn2tm2Sud

Allele Symbol: Nrxn2tm2Sud

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Nrxn1tm2Sud/Nrxn1tm2Sud Nrxn2tm2Sud/Nrxn2tm2Sud Nrxn3tm2Sud/Nrxn3tm2Sudinvolves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

References

Additional References

Additional - Nrxn1tm2Sud related

Additional - Nrxn2tm2Sud related

Additional - Nrxn3tm2Sud related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Nrxn3^tm2Sud

Allele Symbol: Nrxn3^tm2Sud

Nrxn1^tm2Sud

Allele Symbol: Nrxn1^tm2Sud

Nrxn2^tm2Sud

Allele Symbol: Nrxn2^tm2Sud

Genotype: Nrxn1^tm2Sud/Nrxn1^tm2Sud Nrxn2^tm2Sud/Nrxn2^tm2Sud Nrxn3^tm2Sud/Nrxn3^tm2Sud
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Additional - Nrxn1^tm2Sud related

Additional - Nrxn2^tm2Sud related

Additional - Nrxn3^tm2Sud related