B6;D2-Tg(APOE-NPC1L1)20Lqyu/J

Stock number: 008408
Research areas: Internal/Organ Research, Metabolism Research

Description

These transgenic mice express the human NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (NPC1L1) gene under the control of the human apolipoprotein E (APOE) promoter and hepatic control region. This mutant mouse strain exhibits decreased biliary cholesterol levels, which is associated with increased plasma cholesterol levels, and may be useful in studies related to lipid metabolism and cholesterol transport.

Detailed description

These transgenic mice express the human NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (NPC1L1) gene under the control of the human apolipoprotein E (APOE) promoter and hepatic control region. Transgene expression is detected in liver by Western blot analysis and is localized to the canalicular membrane. Transgenic mice exhibit decreased biliary cholesterol levels, which is associated with increased plasma cholesterol levels (mostly apoE-rich HDL particles). Biliary phospholipid and bile acid levels are not affected. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous. This mutant mouse strain may be useful in studies of lipid metabolism and cholesterol transport.

Development

A transgenic construct containing the human NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (NPC1L1) gene under the control of the human apolipoprotein E (APOE) promoter and hepatic control region was injected into fertilized B6D2F1/Hsd mouse eggs. Founder line L1-Tg20 was subsequently established. Founder animals were bred to wildtype B6D2F1/Hsd mice. The Donating Investigator maintained the strain as a hemizygote.

Allele

Symbol: Tg(APOE-NPC1L1)20Lqyu
Name: transgene insertion 20, Liqing Yu
MGI accession ID: MGI:3799059
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(APOE-NPC1L1)20Lqyu
Marker name: transgene insertion 20, Liqing Yu
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F1
Expressed genes: NPC1L1,NPC1 like intracellular cholesterol transporter 1,human
Molecular note: A transgenic construct containing the human NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (NPC1L1) gene under the control of the human apolipoprotein E (APOE) promoter and hepatic control region was injected into fertilized B6D2F1/Hsd mouse eggs. Two lines were established, L1-Tg20 and L1-Tg112. Transgene expression is detected in liver by Western blot analysis and is localized to the canalicular membrane.