These transgenic mice express the human NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (NPC1L1) gene under the control of the human apolipoprotein E (APOE) promoter and hepatic control region. This mutant mouse strain exhibits decreased biliary cholesterol levels, which is associated with increased plasma cholesterol levels, and may be useful in studies related to lipid metabolism and cholesterol transport.
Liqing Yu, Wake Forest Univ. School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These transgenic mice express the human NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (NPC1L1) gene under the control of the human apolipoprotein E (APOE) promoter and hepatic control region. Transgene expression is detected in liver by Western blot analysis and is localized to the canalicular membrane. Transgenic mice exhibit decreased biliary cholesterol levels, which is associated with increased plasma cholesterol levels (mostly apoE-rich HDL particles). Biliary phospholipid and bile acid levels are not affected. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous. This mutant mouse strain may be useful in studies of lipid metabolism and cholesterol transport.
A transgenic construct containing the human NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (NPC1L1) gene under the control of the human apolipoprotein E (APOE) promoter and hepatic control region was injected into fertilized B6D2F1/Hsd mouse eggs. Founder line L1-Tg20 was subsequently established. Founder animals were bred to wildtype B6D2F1/Hsd mice. The Donating Investigator maintained the strain as a hemizygote.
Expressed Gene | NPC1L1, NPC1 like intracellular cholesterol transporter 1, human |
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Site of Expression |
Allele Name | transgene insertion 20, Liqing Yu |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | L1-Tg20; NPC1L1LiverTg; NPCL1-Tg-Mod |
Gene Symbol and Name | Tg(APOE-NPC1L1)20Lqyu, transgene insertion 20, Liqing Yu |
Gene Synonym(s) | |
Promoter | APOE, apolipoprotein E, human |
Expressed Gene | NPC1L1, NPC1 like intracellular cholesterol transporter 1, human |
Strain of Origin | (C57BL/6 x DBA/2)F1 |
Chromosome | UN |
Molecular Note | A transgenic construct containing the human NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (NPC1L1) gene under the control of the human apolipoprotein E (APOE) promoter and hepatic control region was injected into fertilized B6D2F1/Hsd mouse eggs. Two lines were established, L1-Tg20 and L1-Tg112. Transgene expression is detected in liver by Western blot analysis and is localized to the canalicular membrane. |
Mutations Made By | Liqing Yu, Wake Forest Univ. School of Medicine |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator maintained the strain as a hemizygote.
When using the B6;D2-Tg(APOE-NPC1L1)20Lqyu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008408 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(APOE-NPC1L1)20Lqyu |
Frozen Mouse Embryo | B6;D2-Tg(APOE-NPC1L1)20Lqyu/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(APOE-NPC1L1)20Lqyu/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(APOE-NPC1L1)20Lqyu/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;D2-Tg(APOE-NPC1L1)20Lqyu/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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