STOCK Epas1^tm1Mcs/J

Stock number: 008407
Product name: Hif-2α^fl , Hif-2α 2-loxP
Research areas: Neurobiology Research, Research Tools

Description

Hif-2α 2-loxP contains loxP sites flanking exon 2 of the endothelial PAS domain protein 1. These floxed mutant mice may be useful in generating conditional mutations for studies related to erythropoiesis.

Detailed description

The Hif-2α 2-loxP allele, also called Hif-2&alpha^fl or Epas1^2lox, contains loxP sites flanking exon 2 of the endothelial PAS domain protein 1 locus (Epas1 or Hif-2α). Homozygous mice are are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon deleted in the cre-expressing tissue(s).

For example, when bred to a strain with inducible Cre recombinase expression in cardiac cells (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of erythropoiesis.

When bred to a strain with tamoxifen inducible widespread Cre recombinase expression(see Stock No. 008085 for example), this mutant mouse strain may be useful in studies of erythropoiesis.

When bred to a strain expressing inducible Cre recombinase in the osteoblast lineage (Tg(Sp7-tTA,tetO-EGFP/cre)1Amc), see Stock No. 006361 for example), this mutant mouse strain may be useful in studies of erythropoiesis.

Development

A targeting vector was designed to insert a loxP site upstream of exon 2, as well as a loxP-flanked PGK-Neo cassette (in reverse orientation) just downstream of exon 2 of the endothelial PAS domain protein 1 locus (Epas1 or Hif-2α). This construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric animals were bred to EIIa-Cre transgenic mice (on the C57BL/6 genetic background). The resulting offspring with the PGK-Neo cassette removed and exon 2 flanked by loxP sites were identified (and called Hif-2α 2-loxP, Hif-2&alpha^fl or Epas1^2lox). These Hif-2α 2-loxP mice were then bred with wildtype C57BL/6 to remove the EIIa-Cre transgene. Heterozygous Hif-2α 2-loxP mice were bred together to generate homozygotes prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Epas1^tm1Mcs
Name: targeted mutation 1, M Celeste Simon
MGI accession ID: MGI:3526731
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Epas1^2lox; Epas1^fl; Hif-2alpha 2-lox; HIF-2alpha^+f; Hif-2alpha^fl
Marker symbol: Epas1
Marker name: endothelial PAS domain protein 1
Marker synonyms: bHLHe73; ECYT4; Hif like protein; HIF2 alpha; HIF-2 alpha; Hif2a; HIF-2alpha; HLF; HRF; hypoxia inducible transcription factor 2alpha; MOP2; PASD2
Chromosome: 17
Strain of origin: 129X1/SvJ
Molecular note: Exon 2 was flanked with loxP sites. This insertion disrupts putative enhancer Rr343580 and affects the expression of the Atp6v1e2 gene in macrophages.