Hif-2α 2-loxP contains loxP sites flanking exon 2 of the endothelial PAS domain protein 1. These floxed mutant mice may be useful in generating conditional mutations for studies related to erythropoiesis.
M. Celeste Simon, University of Pennsylvania
The Hif-2α 2-loxP allele, also called Hif-2&alphafl or Epas12lox, contains loxP sites flanking exon 2 of the endothelial PAS domain protein 1 locus (Epas1 or Hif-2α). Homozygous mice are are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon deleted in the cre-expressing tissue(s).
For example, when bred to a strain with inducible Cre recombinase expression in cardiac cells (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of erythropoiesis.
When bred to a strain with tamoxifen inducible widespread Cre recombinase expression(see Stock No. 008085 for example), this mutant mouse strain may be useful in studies of erythropoiesis.
When bred to a strain expressing inducible Cre recombinase in the osteoblast lineage (Tg(Sp7-tTA,tetO-EGFP/cre)1Amc), see Stock No. 006361 for example), this mutant mouse strain may be useful in studies of erythropoiesis.
A targeting vector was designed to insert a loxP site upstream of exon 2, as well as a loxP-flanked PGK-Neo cassette (in reverse orientation) just downstream of exon 2 of the endothelial PAS domain protein 1 locus (Epas1 or Hif-2α). This construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric animals were bred to EIIa-Cre transgenic mice (on the C57BL/6 genetic background). The resulting offspring with the PGK-Neo cassette removed and exon 2 flanked by loxP sites were identified (and called Hif-2α 2-loxP, Hif-2&alphafl or Epas12lox). These Hif-2α 2-loxP mice were then bred with wildtype C57BL/6 to remove the EIIa-Cre transgene. Heterozygous Hif-2α 2-loxP mice were bred together to generate homozygotes prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, M Celeste Simon|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Epas12lox; Epas1fl; Hif-2alpha 2-lox; HIF-2alpha+f; Hif-2alphafl|
|Gene Symbol and Name||Epas1, endothelial PAS domain protein 1|
|Strain of Origin||129X1/SvJ|
|Molecular Note||Exon 2 was flanked with loxP sites.|
|Mutations Made By|| |
M. Celeste Simon, University of Pennsylvania
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Hif-2αfl , Hif-2α 2-loxP mouse strain in a publication, please cite the originating article(s) and include JAX stock #008407 in your Materials and Methods section.
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