This targeted mutation strain carries a deletion of exons 2 and 3 of the neuroligin 3 (NLGN3) gene. These mice show no alteration in their inhibitory synaptic transmission characteristics. Homozygotes are viable, normal in size and do not display any gross physical abnormalities. This mutant mouse strain may be useful in studies of synapse formation and/or function.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Nlgn3 | neuroligin 3 |
This targeted mutation strain carries a deletion of exons 2 and 3 of the gene. These mice show no alteration in their inhibitory synaptic transmission characteristics. No protein product is detected by Western blot analysis of homozygous brain extracts. Homozygotes are viable, normal in size and do not display any gross physical abnormalities. This mutant mouse strain may be useful in studies of synapse formation and/or function.
Exons 2 and 3 were deleted using Cre-lox and FLP-FRT technologies. The targeting vector was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. This line was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.
Allele Name | targeted mutation 2.1, Thomas C Sudhof |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Nlgn3, neuroligin 3 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | X |
Molecular Note | Exons 2 and 3 were removed by cre-mediated recombination that also removed the neo cassette. The loss of protein expression was confirmed by western blot analysis using two antibodies (528B and 639B). |
Mutations Made By | Dr. Thomas Sudhof, Stanford University School of Medicine |
When maintained as a live colony, homozygous females, heterozygous females or hemizygous males may be bred. This gene/mutation is X-linked.
When using the NL3 mouse strain in a publication, please cite the originating article(s) and include JAX stock #008394 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X linked - Heterozygous females and Wild-type Males for Nlgn3<tm2.1Sud> |
Frozen Mouse Embryo | B6;129-Nlgn3<tm2.1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Nlgn3<tm2.1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Nlgn3<tm2.1Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Nlgn3<tm2.1Sud>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.