B6;129-Nlgn3^tm2.1Sud/J

Stock number: 008394
Product name: NL3
Research areas: Developmental Biology Research, Neurobiology Research

Description

This targeted mutation strain carries a deletion of exons 2 and 3 of the neuroligin 3 (NLGN3) gene. These mice show no alteration in their inhibitory synaptic transmission characteristics. Homozygotes are viable, normal in size and do not display any gross physical abnormalities. This mutant mouse strain may be useful in studies of synapse formation and/or function.

Detailed description

This targeted mutation strain carries a deletion of exons 2 and 3 of the gene. These mice show no alteration in their inhibitory synaptic transmission characteristics. No protein product is detected by Western blot analysis of homozygous brain extracts. Homozygotes are viable, normal in size and do not display any gross physical abnormalities. This mutant mouse strain may be useful in studies of synapse formation and/or function.

Development

Exons 2 and 3 were deleted using Cre-lox and FLP-FRT technologies. The targeting vector was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. This line was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.

Allele

Symbol: Nlgn3^tm2.1Sud
Name: targeted mutation 2.1, Thomas C Sudhof
MGI accession ID: MGI:3758960
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Nlgn3
Marker name: neuroligin 3
Chromosome: X
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exons 2 and 3 were removed by cre-mediated recombination that also removed the neo cassette. The loss of protein expression was confirmed by western blot analysis using two antibodies (528B and 639B).