JAX Mouse Strain Datasheet - 008394

B6;129-Nlgn3^tm2.1Sud/J

Stock No:: 008394

Targeted Mutation

Cryo Recovery

Overview

This targeted mutation strain carries a deletion of exons 2 and 3 of the neuroligin 3 (NLGN3) gene. These mice show no alteration in their inhibitory synaptic transmission characteristics. Homozygotes are viable, normal in size and do not display any gross physical abnormalities. This mutant mouse strain may be useful in studies of synapse formation and/or function.

Donating Investigator

Dr. Thomas C. Sudhof, Stanford University School of Medicine

Genetic overview

Genetic Background	Generation

Nlgn3^tm2.1Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Nlgn3	neuroligin 3

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Research Applications

Developmental Biology Research
Neurobiology Research

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Base Price

Starting at:

$2,225.00 Domestic price Cryo Recovery

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Details

Detailed Description

This targeted mutation strain carries a deletion of exons 2 and 3 of the gene. These mice show no alteration in their inhibitory synaptic transmission characteristics. No protein product is detected by Western blot analysis of homozygous brain extracts. Homozygotes are viable, normal in size and do not display any gross physical abnormalities. This mutant mouse strain may be useful in studies of synapse formation and/or function.

Development

Exons 2 and 3 were deleted using Cre-lox and FLP-FRT technologies. The targeting vector was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. This line was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.

Selected References

Tabuchi K; Blundell J; Etherton MR; Hammer RE; Liu X; Powell CM; Sudhof TC. 2007. A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice. Science 318(5847):71-6. PubMed: 17823315 MGI: J:125344

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Genetics

Nlgn3^tm2.1Sud

Allele Symbol: Nlgn3^tm2.1Sud

Allele Name	targeted mutation 2.1, Thomas C Sudhof
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Nlgn3, neuroligin 3
Gene Synonym(s)	A230085M13Rik; A230085M13Rik; HNL3; NL3; RIKEN cDNA A230085M13 gene
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Chromosome	X
Molecular Note	Exons 2 and 3 were removed by cre-mediated recombination that also removed the neo cassette. The loss of protein expression was confirmed by western blot analysis using two antibodies (528B and 639B).
Mutations Made By	Dr. Thomas Sudhof, Stanford University School of Medicine

Disease/Phenotype

Disease Terms

Autism, Susceptibility to, X-Linked 1; AUTSX1

Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.

Asperger Syndrome, X-Linked, Susceptibility to, 1; ASPGX1 (NLGN3)

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Neurodevelopmental Defects
Neurobiology Research
- Neurodevelopmental Defects
  - Autism

Mammalian Phenotype Terms by Genotype

Genotype: Nlgn3^tm2.1Sud/Y
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

no abnormal phenotype detected
- mice are viable, fertile, have no obvious abnormalities, and exhibit normal neurological and behavioral phenotypes

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Nlgn3^tm2.1Sud/Y
involves: 129S1/Sv * 129X1/SvJ

behavior/neurological phenotype

enhanced learning
- mice exhibit increased learning rate but similar initial coordination on the accelerating rotarod

hyperactivity
- mice are hyperactive during open-field tests, showing increased total distance traveled and increased number of ambulatory episodes, however no changes in movement velocity or in crossings through the center of the open field are seen
- mice exhibit greater number of wall jumps

increased stereotypic behavior
- mice perform similarly to wild-type littermates on the first trial of an accelerating rotarod, however in the last several trials, mice exhibit enhanced performance, with increased time on the rotarod before falling off compared to wild-type mice, indicating enhanced formation of repetitive motor routineing enhanced formation of repetitive motor routine
- mice show increased vigor of stereotypic movements, indicated by greater force variance during periods of low mobility
- the time spend performing stereotypic movements is enhanced during spontaneous open-field activity

stereotypic behavior
- mice show an abnormal bias in the direction of rotation during locomotion

References

Tabuchi K; Blundell J; Etherton MR; Hammer RE; Liu X; Powell CM; Sudhof TC. 2007. A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice. Science 318(5847):71-6. PubMed: 17823315 MGI: J:125344

Additional - Nlgn3^tm2.1Sud related

Rothwell PE; Fuccillo MV; Maxeiner S; Hayton SJ; Gokce O; Lim BK; Fowler SC; Malenka RC; Sudhof TC. 2014. Autism-associated neuroligin-3 mutations commonly impair striatal circuits to boost repetitive behaviors. Cell 158(1):198-212. PubMed: 24995986 MGI: J:214636

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Please inquire about possible genotypes.

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Email: tjlca@jax.org

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Selected References

Nlgn3tm2.1Sud

Allele Symbol: Nlgn3tm2.1Sud

Disease Terms

Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Nlgn3tm2.1Sud/Yinvolves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

Genotype: Nlgn3tm2.1Sud/Yinvolves: 129S1/Sv * 129X1/SvJ

behavior/neurological phenotype

References

Additional - Nlgn3tm2.1Sud related

Genotyping Protocols

Breeding Considerations

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Nlgn3^tm2.1Sud

Allele Symbol: Nlgn3^tm2.1Sud

Genotype: Nlgn3^tm2.1Sud/Y
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Genotype: Nlgn3^tm2.1Sud/Y
involves: 129S1/Sv * 129X1/SvJ

Additional - Nlgn3^tm2.1Sud related