Thymocytes from homozygous targeted mutation mice are partially protected from anti-CD3 mAb-mediated deletion in vivo and in vitro. No obvious abnormalities in the lymphoid compartments have been observed. No expression was detected in splenocytes assayed by Western blot. Homozygotes are viable, fertile and have no breeding difficulties. This strain may be useful in studies of T-cell apoptosis.
Dr. Richard A. Flavell, Yale University School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Map2k6 | mitogen-activated protein kinase kinase 6 |
Thymocytes from homozygous targeted mutation mice are partially protected from anti-CD3 mAb-mediated deletion in vivo and in vitro. No obvious abnormalities in the lymphoid compartments have been observed. No expression was detected in splenocytes assayed by Western blot. Homozygotes are viable, fertile and have no breeding difficulties. This strain may be useful in studies of T-cell apoptosis.
Subdomain XI, a catalytic domain required for gene activation, was replaced with a neomycin resistance cassette. The targeting vector was electroporated into 129S1/Sv Oca2+ Tyr+ Kitl +-derived W9.5 embryonic stem (ES) cells. This strain was backcrossed ten times to C57BL/6Ncr by the donating laboratory.
Allele Name | targeted mutation 1, Richard A Flavell |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Mkk6- |
Gene Symbol and Name | Map2k6, mitogen-activated protein kinase kinase 6 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 11 |
Molecular Note | A neo cassette inserted by homologous recombination replaced the exon encoding subdomain XI which is essential for catalytic activity. Protein was undetected by Western blot analaysis of splenocytes obtained from homozygous mutant mice. |
Mutations Made By | Dr. Richard Flavell, Yale University School of Medicine |
When maintained as a live colony, homozygotes may be bred.
When using the B6.129S1-Map2k6tm1Flv/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008382 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Map2k6<tm1Flv> |
Frozen Mouse Embryo | B6.129S1-Map2k6<tm1Flv>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Map2k6<tm1Flv>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Map2k6<tm1Flv>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Map2k6<tm1Flv>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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