B6.129S1-Map2k6^tm1Flv/J

Stock number: 008382
Research areas: Apoptosis Research, Immunology, Inflammation and Autoimmunity Research

Description

Thymocytes from homozygous targeted mutation mice are partially protected from anti-CD3 mAb-mediated deletion in vivo and in vitro. No obvious abnormalities in the lymphoid compartments have been observed. No expression was detected in splenocytes assayed by Western blot. Homozygotes are viable, fertile and have no breeding difficulties. This strain may be useful in studies of T-cell apoptosis.

Detailed description

Thymocytes from homozygous targeted mutation mice are partially protected from anti-CD3 mAb-mediated deletion in vivo and in vitro. No obvious abnormalities in the lymphoid compartments have been observed. No expression was detected in splenocytes assayed by Western blot. Homozygotes are viable, fertile and have no breeding difficulties. This strain may be useful in studies of T-cell apoptosis.

Development

Subdomain XI, a catalytic domain required for gene activation, was replaced with a neomycin resistance cassette. The targeting vector was electroporated into 129S1/Sv Oca2⁺ Tyr⁺ Kitl ⁺-derived W9.5 embryonic stem (ES) cells. This strain was backcrossed ten times to C57BL/6Ncr by the donating laboratory.

Allele

Symbol: Map2k6^tm1Flv
Name: targeted mutation 1, Richard A Flavell
MGI accession ID: MGI:2673716
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Map2k6
Marker name: mitogen-activated protein kinase kinase 6
Chromosome: 11
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: A neo cassette inserted by homologous recombination replaced the exon encoding subdomain XI which is essential for catalytic activity. Protein was undetected by Western blot analaysis of splenocytes obtained from homozygous mutant mice.