Cells from these Myo1f (myosin IF) targeted mutant mice exhibit abnormally increased immune cell adhesion and reduced motility, resulting from augmented exocytosis of B2 integrin-containing granules. Also, the cortical actin that normally co-localizes with this gene's product is reduced in homozygous targeted mice. The animals show increased in vivo susceptibility to infection by Listeria monocytogenes and an impaired neutrophil response. This strain may be useful in studies of immune cell motility and innate host defence against infection.
Dr. Richard A. Flavell, Yale University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Myo1f | myosin IF |
Cells from these targeted mutant mice exhibit abnormally increased immune cell adhesion and reduced motility, resulting from augmented exocytosis of β2 integrin-containing granules. Also, the cortical actin that normally co-localizes with this gene's product is reduced in homozygous targeted mice. The animals show increased in vivo susceptibility to infection by Listeria monocytogenes and an impaired neutrophil response. This strain may be useful in studies of immune cell motility and innate host defence against infection. Homozygous targeted mice are viable, fertile and have no obvious developmental defects.
A targeting vector carrying a loxP-flanked neomycin resistance gene in intron 6 and an additional loxP site in intron 4 was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Cre treatment of the targeted ES cell clones generated the mutant allele by deleting exons 5, 6, and the neomycin gene. This strain was backcrossed 10 times to C57BL/6Ncr by the donating laboratory.
Allele Name | targeted mutation 1.1, Richard A Flavell |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Myo1f, myosin IF |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 17 |
Molecular Note | Exons 5 and 6 were deleted from the locus via cre mediated recombination. Absense of protein was confirmed by Western blot analysis. |
Mutations Made By | Dr. Richard Flavell, Yale University School of Medicine |
When maintained as a live colony, homozygotes may be bred.
When using the B6.129S6-Myo1ftm1.1Flv/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008381 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Myo1f<tm1.1Flv> |
Frozen Mouse Embryo | B6.129S6-Myo1f<tm1.1Flv>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Myo1f<tm1.1Flv>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Myo1f<tm1.1Flv>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Myo1f<tm1.1Flv>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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