B6;129S4-Trp53^tm5Tyj/J

Stock number: 008361
Research areas: Cancer Research, Research Tools

Description

These targeted mutant mice carry a loxP-flanked STOP cassette in intron 1 that blocks expression of the Trp53, transformation related protein 53 gene. Both heterozygotes and homozygotes are prone to the development of tumors (primarily spontaneous thymic lymphomas and sarcomas). Upon Cre-mediated recombination, the STOP cassette can be excised, leading to restoration of gene expression. This strain permits temporal control of this tumor suppressor gene, and enables studies of tumorigenesis.

Detailed description

These targeted mutant mice carry a loxP-flanked STOP cassette in intron 1 that blocks expression of the gene. Both heterozygotes and homozygotes are prone to the development of tumors (primarily spontaneous thymic lymphomas and sarcomas). The latency is significantly shorter in homozygotes than in wildtype mice. Homozygotes have reduced fertility. Upon Cre-mediated recombination, the STOP cassette can be excised, leading to restoration of gene expression. This strain permits temporal control of this tumor suppressor gene, and enables studies of tumorigenesis.

Development

A loxP-flanked STOP cassette was introduced to intron 1 to block expression of the gene. The targeting vector was introduced to 129S4/SvJae-derived J1 embryonic stem (ES) cells. This line was maintained on a mixed 129S4/SvJae and C57BL/6 background by the donating investigator.

Allele

Symbol: Trp53^tm5Tyj
Name: targeted mutation 5, Tyler Jacks
MGI accession ID: MGI:3702077
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Trp53
Marker name: transformation related protein 53
Chromosome: 11
Strain of origin: 129S4/SvJae
Molecular note: A lox-stop-lox cassette is integrated into intron 1 disrupting expression of the gene.