These targeted mutant mice carry a loxP-flanked STOP cassette in intron 1 that blocks expression of the Trp53, transformation related protein 53 gene. Both heterozygotes and homozygotes are prone to the development of tumors (primarily spontaneous thymic lymphomas and sarcomas). Upon Cre-mediated recombination, the STOP cassette can be excised, leading to restoration of gene expression. This strain permits temporal control of this tumor suppressor gene, and enables studies of tumorigenesis.
Dr. Tyler Jacks, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Trp53 | transformation related protein 53 |
These targeted mutant mice carry a loxP-flanked STOP cassette in intron 1 that blocks expression of the gene. Both heterozygotes and homozygotes are prone to the development of tumors (primarily spontaneous thymic lymphomas and sarcomas). The latency is significantly shorter in homozygotes than in wildtype mice. Homozygotes have reduced fertility. Upon Cre-mediated recombination, the STOP cassette can be excised, leading to restoration of gene expression. This strain permits temporal control of this tumor suppressor gene, and enables studies of tumorigenesis.
A loxP-flanked STOP cassette was introduced to intron 1 to block expression of the gene. The targeting vector was introduced to 129S4/SvJae-derived J1 embryonic stem (ES) cells. This line was maintained on a mixed 129S4/SvJae and C57BL/6 background by the donating investigator.
Allele Name | targeted mutation 5, Tyler Jacks |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | p53f; p53LSL; p53LSL-WT; p53-LSL; Trp53LSL |
Gene Symbol and Name | Trp53, transformation related protein 53 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 11 |
Molecular Note | A lox-stop-lox cassette is integrated into intron 1 disrupting expression of the gene. |
Mutations Made By | Dr. Tyler Jacks, Massachusetts Institute of Technology |
When maintained as a live colony, heterozygotes or homozygotes may be bred. Homozygous fertility is slightly reduced. Both heterozygotes and homozygotes are prone to tumor development.
When using the B6;129S4-Trp53tm5Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008361 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Trp53<tm5Tyj> |
Frozen Mouse Embryo | B6;129S4-Trp53<tm5Tyj>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Trp53<tm5Tyj>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Trp53<tm5Tyj>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Trp53<tm5Tyj>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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