Ptpn6f mice harbor loxP sites flanking teh region including exon 1 through most of exon 9 of the Ptpn6 (protein tyrosine phosphatase, non-receptor type 6; also called Shp1) gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). These Ptpn6f mice may be useful in generating conditional mutations for studying the role of Ptpn6 in inflammation and immunology research.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Ptpn6 | protein tyrosine phosphatase, non-receptor type 6 |
Mice homozygous for the Ptpn6f allele are viable and fertile, with loxP sites flanking exon 1(II) through most of exon 9 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in cre-expressing tissue(s). These Ptpn6f mice may be useful in generating conditional mutations for studying the role of Ptpn6 (Shp1) in inflammation and immunology research.
For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studying the motheaten (me) phenotype; characterized by widespread inflammation and autoimmunity.
When bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126, Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte development.
A targeting vector was designed to insert a loxP-flanked neomycin resistance cassette in the 5' UTR of exon 1(II), and a third loxP site in intron 9 of the targeted gene. This construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre-expressing plasmid to remove the selection cassette. ES cells in which the neomycin resistance cassette was deleted (Ptpn6f; leaving a single loxP site in the 5' UTR and a loxP within intron 9) were injected into recipient blastocysts. Chimeric males were bred with C57BL/6 females to generate Ptpn6f mice. These Ptpn6f mice were subsequently backcrossed to C57BL/6 for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to C57BL/6J for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Klaus Rajewsky |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Ptpn6f; SHP-1Flox |
Gene Symbol and Name | Ptpn6, protein tyrosine phosphatase, non-receptor type 6 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 6 |
Molecular Note | A vector was designed to flank exon 1 through exon 9 with loxP sites. A floxed neo was removed via transient cre expression. |
Mutations Made By | Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129P2-Ptpn6tm1Rsky/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008336 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Ptpn6<tm1Rsky> |
Frozen Mouse Embryo | B6.129P2-Ptpn6<tm1Rsky>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Ptpn6<tm1Rsky>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Ptpn6<tm1Rsky>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Ptpn6<tm1Rsky>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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