B6.129P2-Ptpn6^tm1Rsky/J

Stock number: 008336
Research areas: Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Ptpn6^f mice harbor loxP sites flanking teh region including exon 1 through most of exon 9 of the Ptpn6 (protein tyrosine phosphatase, non-receptor type 6; also called Shp1) gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). These Ptpn6^f mice may be useful in generating conditional mutations for studying the role of Ptpn6 in inflammation and immunology research.

Detailed description

Mice homozygous for the Ptpn6^f allele are viable and fertile, with loxP sites flanking exon 1(II) through most of exon 9 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in cre-expressing tissue(s). These Ptpn6^f mice may be useful in generating conditional mutations for studying the role of Ptpn6 (Shp1) in inflammation and immunology research.

For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studying the motheaten (me) phenotype; characterized by widespread inflammation and autoimmunity.

When bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126, Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte development.

Development

A targeting vector was designed to insert a loxP-flanked neomycin resistance cassette in the 5' UTR of exon 1(II), and a third loxP site in intron 9 of the targeted gene. This construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre-expressing plasmid to remove the selection cassette. ES cells in which the neomycin resistance cassette was deleted (Ptpn6^f; leaving a single loxP site in the 5' UTR and a loxP within intron 9) were injected into recipient blastocysts. Chimeric males were bred with C57BL/6 females to generate Ptpn6^f mice. These Ptpn6^f mice were subsequently backcrossed to C57BL/6 for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to C57BL/6J for at least one generation to establish the colony.

Allele

Symbol: Ptpn6^tm1Rsky
Name: targeted mutation 1, Klaus Rajewsky
MGI accession ID: MGI:3719625
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ptpn6
Marker name: protein tyrosine phosphatase, non-receptor type 6
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Molecular note: A vector was designed to flank exon 1 through exon 9 with loxP sites. A floxed neo was removed via transient cre expression.