These mutant mice carry a His6-tagged myelocytomatosis oncogene (Myc) gene (cDNA) sequence inserted in the endogenous immunoglobulin heavy chain complex (Igh) locus, which mimics the human endemic Burkitt lymphoma t(8;14)(q24;q32) translocation and mouse plasmacytoma T(12;15) translocation. This mutant mouse strain may be useful in studies of Burkitt Lymphoma and plasma cell and B-cell neoplasia.
Siegfried Janz, University of Iowa
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Inserted expressed sequence, Humanized sequence) | Igh | immunoglobulin heavy chain complex |
These mutant mice carry a His6-tagged Myc gene (cDNA) sequence inserted in the endogenous immunoglobulin heavy chain complex (Igh) locus, which mimics the human endemic Burkitt lymphoma t(8;14)(q24;q32) translocation and mouse plasmacytoma T(12;15) translocation.
The His6-tagged Myc is overexpressed in B-cells throughout B-cell development, as detected by FACS analysis. Tumor-free heterozygotes, 4 to 6 months of age, on a mixed B6;129X1 background, exhibit increased B-cell proliferation and apoptosis and have enlarged lymph nodes and spleen due to follicular hyperplasia. 68% of mutant mice between 6 and 21 months of age develop mature B-cell tumors that are IgM and BCL6 positive. Approximately half of the tumors detected are lymphoblastic B-cell lymphomas that are Burkitt-like in appearance and one fifth as plasmacytomas. The plasmacytomas occur in gut-associated lymphoid tissue (GALT), especially in the mesenteric lymph node and Peyer's patches. Mice that are homozygous for the targeted mutation are viable and fertile.
This mutant mouse strain may be useful in studies of Burkitt Lymphoma and B-cell and plasma cell neoplasia.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a floxed neo selection cassette, His6 (an artificial
histidine tag) and sequence encoding the mouse Myc gene was inserted 5' to the E-mu intronic enhancer of the Igh locus.
The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells.
Correctly targeted ES cells were injected into C57BL/6 blastocysts.
The resulting chimeric animals were crossed to transgenic Cre recombinase expressing mice to remove the neo selection cassette. The mice were then backcrossed to BALB/cAnPt for 13 generations.
Expressed Gene | Myc, myelocytomatosis oncogene, mouse, laboratory |
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Site of Expression |
Allele Name | targeted mutation 1, Siegfried Janz |
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Allele Type | Targeted (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | iMycEmu |
Gene Symbol and Name | Igh, immunoglobulin heavy chain complex |
Gene Synonym(s) | |
Expressed Gene | Myc, myelocytomatosis oncogene, mouse, laboratory |
Strain of Origin | Not Specified |
Chromosome | 12 |
Molecular Note | A His6-tagged Myc cDNA was inserted head to head into the locus 5' of the intronic enhancer Emu. This insertion of Myc mimics both the human t(8;14)(q24;q32) translocation that results in the activation of MYC in human endemic Burkitt lymphomas and the homologous mouse T(12;15) translocation that deregulates Myc in mouse plasmacytomas. |
Mutations Made By | Siegfried Janz, University of Iowa |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the C.129S1-Ightm1(Myc)Janz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008332 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Igh<tm1Janz> |
Frozen Mouse Embryo | C.129S1-Igh<tm1(Myc)Janz>/J | $2595.00 |
Frozen Mouse Embryo | C.129S1-Igh<tm1(Myc)Janz>/J | $2595.00 |
Frozen Mouse Embryo | C.129S1-Igh<tm1(Myc)Janz>/J | $3373.50 |
Frozen Mouse Embryo | C.129S1-Igh<tm1(Myc)Janz>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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