FVB-Tg(Pomc-rtTA)1Rck/J

Stock number: 008326
Research areas: Neurobiology Research, Research Tools

Description

A pro-opiomelanocortin-alpha (Pomc) promoter drives expression of a modified reverse tetracycline regulatable transactivator (rtTA2^S-M2_{F86Y A209T}) in this Tet-On transgenic strain. When these mice are mated to a second transgenic strain carrying a target gene regulated by the tetO responsive elements, expression of the target gene is turned on in POMC-expressing neurons by the tetracycline analog doxycycline (dox).

Detailed description

The mouse pro-opiomelanocortin-alpha (Pomc) promoter drives expression of a modified reverse tetracycline regulatable transactivator (rtTA2^S-M2_{F86Y A209T}) in this Tet-On transgenic strain. When these mice are mated to a second transgenic strain carrying a target gene regulated by the tetO responsive elements, expression of the target gene is turned on in POMC-expressing neurons by the tetracycline analog doxycycline (dox). Dox can be administered orally in the food or water. Hemizygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

Development

A bacterial artificial chromosome (BAC) containing the mouse pro-opiomelanocortin-alpha (Pomc) gene was modified so that the Pomc promoter drives expression of a modified reverse tetracycline regulatable transactivator (rtTA2^S-M2_{F86Y A209T}; Tet-ON transactivator). This strain was created and maintained on an FVB background by the donating laboratory.

Allele

Symbol: Tg(Pomc-rtTA)1Rck
Name: transgene insertion 1, Jeffrey M Friedman
MGI accession ID: MGI:3785562
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Pomc-rtTA)1Rck
Marker name: transgene insertion 1, Jeffrey M Friedman
Chromosome: UN
Strain of origin: FVB
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: When mice carryint this transgene are mated to a second transgenic strain carrying a target gene regulated by the tetO responsive elements, expression of the target gene is turned on in POMC-expressing neurons by the tetracycline analog doxycycline.
Molecular note: A bacterial artificial chromosome (BAC) containing the mouse pro-opiomelanocortin-alpha (Pomc) gene was modified so that the Pomc promoter drives expression of reverse tetracycline regulatable transactivator (rtTA; Tet-ON transactivator).