B6.Cg-Tg(Pmch-MAPT/CFP)1Rck/J

Stock number: 008324
Strain synonyms: Tg(Pmch-MAPT/Topaz)1Rck
Research areas: Neurobiology Research, Research Tools

Description

These mice express a tau (MAPT)-cyan fluorescent protein (CFP) under the transcriptional control of the mouse pro-melanin-concentrating hormone (Pmch) promoter. Neurons expressing PMCH can be easily identified in the brain. Hemizygotes are viable and fertile and do not display any gross physical or behavioral abnormalities.

Detailed description

These mice express a tau (MAPT)-cyan fluorescent protein (CFP) under the transcriptional control of the mouse pro-melanin-concentrating hormone (Pmch) promoter. Neurons expressing PMCH can be easily identified in the brain. Hemizygotes are viable and fertile and do not display any gross physical or behavioral abnormalities.

Development

A transgenic BAC vector carrying Tau (MAPT)-Cyan fluorescent protein downstream of the ATG sequence of the mouse pro-melanin-concentrating hormone (Pmch) gene was introduced to B6CBAF1 embryos. The line was backcrossed more than ten times to C57BL/6 by the donating laboratory.

Allele

Symbol: Tg(Pmch-MAPT/CFP)1Rck
Name: transgene insertion 1, Jeffrey M Friedman
MGI accession ID: MGI:3785545
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Pmch-MAPT/CFP)1Rck
Marker name: transgene insertion 1, Jeffrey M Friedman
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: MAPT,microtubule associated protein tau,human; GFP,Green Fluorescent Protein,
Site of expression: in neurons that express pro-melanin-concentrating hormone
Molecular note: A transgenic BAC vector carrying Tau (MAPT)-Cyan GFP downstream of the ATG sequence of the mouse pro-melanin-concentrating hormone (Pmch) gene was introduced into fertilized embryos.