B6.Cg-Tg(Npy-MAPT/Sapphire)1Rck/J

Stock number: 008321
Research areas: Diabetes and Obesity Research, Neurobiology Research, Research Tools

Description

These mice express a tau (MAPT)-sapphire green fluorescent protein (GFP) under the transcriptional control of the mouse neuropeptide Y (Npy) promoter. Fluorescence is observed in distinct neuronal populations of the hypothalamic arcuate nucleus. This strain has been useful in studying the role of neuropeptide Y and leptin in food intake. Hemizygotes are viable and fertile and do not display any gross physical or behavioral abnormalities.

Detailed description

These mice express a tau (MAPT)-sapphire green fluorescent protein (GFP) under the transcriptional control of the mouse neuropeptide Y (Npy) promoter. Immunohistochemistry for NPY in colchicine-treated animals demonstrated ~95% colocalization of NPY with the GFP-expressing neurons in the hypothalamic arcuate nucleus. This strain has been useful in studying the role of neuropeptide Y and leptin in food intake. Hemizygotes are viable and fertile and do not display any gross physical or behavioral abnormalities.

Development

A transgenic BAC vector carrying Tau (MAPT)-Sapphire GFP in exon 2 of a mouse neuropeptide Y (Npy) gene was introduced to B6CBAF1 embryos. The line was backcrossed ten times to C57BL/6 by the donating laboratory.

Allele

Symbol: Tg(Npy-MAPT/Sapphire)1Rck
Name: transgene insertion 1, Jeffrey M Friedman
MGI accession ID: MGI:3785480
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Npy-MAPT/Sapphire)1Rck
Marker name: transgene insertion 1, Jeffrey M Friedman
Chromosome: UN
Strain of origin: (CBA x C57BL/6)F1
Expressed genes: GFP,Green Fluorescent Protein,; MAPT,microtubule associated protein tau,human
Site of expression: Fluorescence is observed in distinct neuronal populations of the hypothalamic arcuate nucleus.
Molecular note: A transgenic BAC vector carrying Tau (MAPT)-Sapphire GFP in exon 2 of a mouse neuropeptide Y (Npy) gene was introduced to fertilized embryos