B6.129(Cg)-Lepr^tm2(cre)Rck/J

Stock number: 008320
Product name: ObRb-Cre
Research areas: Diabetes and Obesity Research, Neurobiology Research, Research Tools

Description

Cre activity is demonstrable in the hypothalamus, limbic and cortical brain regions, and retrosplenial cortex of these knock-in Lepr mice. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express the targeted gene. This strain has been used in virus-assisted mapping of neural inputs and may be useful in studies of neural features of feeding behaviors.

Detailed description

Mice heterozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Cre activity is demonstrable in the hypothalamus (arcuate, dorsomedial (DMH), lateral (LH), and ventromedial (VMH) nuclei), limbic and cortical brain regions (basolateral amygdaloid nucleus (BLA), piriform cortex (Pir), and lateral entorhinal cortex (LEnt)), and retrosplenial cortex. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express the gene. This strain has been used in virus-assisted mapping of neural inputs and may be useful in studies of neural features of feeding behaviors.

Development

The targeting vector containing an IRES (internal ribosome entry site)-NLS(nuclear localization signal)-Cre and a neomycin resistance gene (flanked by frt sites) was inserted immediately 3' of the stop codon in the last exon of the gene. The vector was introduced to a 129-background embryonic stem (ES) cell line. This strain was backcrossed ten times to C57BL/6 background by the donating laboratory.

Recent genotyping (March, 2016) indicates that the neomycin allele has been excised from this strain.

Allele

Symbol: Lepr^tm2(cre)Rck
Name: targeted mutation 2, Jeffrey M Friedman
MGI accession ID: MGI:3776158
Generation method: Targeted
Attributes: Recombinase-expressing
Synonyms: Lepr^cre; LepRb^Cre; LeprB-Cre; LepRb-IRES-Cre; ObRb-Cre; Obrb-IRES-Cre
Marker symbol: Lepr
Marker name: leptin receptor
Marker synonyms: CD295; Fa; LEP-R; Leprb; LEPRD; LEPROT; leptin receptor gene-related protein; Modb1; obese-like; obl; Obr; OB-R; OB-RGRP
Chromosome: 4
Strain of origin: Not Specified
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre activity is demonstrable in the hypothalamus (arcuate, dorsomedial, lateral, and ventromedial nuclei), limbic and cortical brain regions (basolateral amygdaloid nucleus , piriform cortex, and lateral entorhinal cortex), and retrosplenial cortex.
Molecular note: A cre recombinase expression cassette containing an IRES domain and a FRT-flanked neomcyin cassette was placed immediately downstream of the stop codon for the gene. Flp-mediated recombination removed the FRT-flanked neo cassette. Cre expression in the hypothalamus was confirmed by use of a virus containing a cre-dependent reporter gene.