Cre activity is demonstrable in the hypothalamus, limbic and cortical brain regions, and retrosplenial cortex of these knock-in Lepr mice. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express the targeted gene. This strain has been used in virus-assisted mapping of neural inputs and may be useful in studies of neural features of feeding behaviors.
Jeffrey Friedman, Rockefeller University
Genetic Background | Generation |
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N11+pN3F10
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Lepr | leptin receptor |
Mice heterozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Cre activity is demonstrable in the hypothalamus (arcuate, dorsomedial (DMH), lateral (LH), and ventromedial (VMH) nuclei), limbic and cortical brain regions (basolateral amygdaloid nucleus (BLA), piriform cortex (Pir), and lateral entorhinal cortex (LEnt)), and retrosplenial cortex. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express the gene. This strain has been used in virus-assisted mapping of neural inputs and may be useful in studies of neural features of feeding behaviors.
The targeting vector containing an IRES (internal ribosome entry site)-NLS(nuclear localization signal)-Cre and a neomycin resistance gene (flanked by frt sites) was inserted immediately 3' of the stop codon in the last exon of the gene. The vector was introduced to a 129-background embryonic stem (ES) cell line. This strain was backcrossed ten times to C57BL/6 background by the donating laboratory.
Recent genotyping (March, 2016) indicates that the neomycin allele has been excised from this strain.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre activity is demonstrable in the hypothalamus (arcuate, dorsomedial, lateral, and ventromedial nuclei), limbic and cortical brain regions (basolateral amygdaloid nucleus , piriform cortex, and lateral entorhinal cortex), and retrosplenial cortex. |
Allele Name | targeted mutation 2, Jeffrey M Friedman |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Leprcre; LepRbCre; LepRb-IRES-Cre; ObRb-Cre; Obrb-IRES-Cre |
Gene Symbol and Name | Lepr, leptin receptor |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre activity is demonstrable in the hypothalamus (arcuate, dorsomedial, lateral, and ventromedial nuclei), limbic and cortical brain regions (basolateral amygdaloid nucleus , piriform cortex, and lateral entorhinal cortex), and retrosplenial cortex. |
Strain of Origin | Not Specified |
Chromosome | 4 |
Molecular Note | A cre recombinase expression cassette containing an IRES domain and a FRT-flanked neomcyin cassette was placed immediately downstream of the stop codon for the gene. Flp-mediated recombination removed the FRT-flanked neo cassette. Cre expression in the hypothalamus was confirmed by use of a virus containing a cre-dependent reporter gene. |
Mutations Made By | Jeffrey Friedman, Rockefeller University |
When maintained as a live colony, heterozygotes may be bred.
When using the ObRb-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #008320 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for |
Frozen Mouse Embryo | B6.129(Cg)-Lepr<tm2(cre)Rck>/J | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Lepr<tm2(cre)Rck>/J | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Lepr<tm2(cre)Rck>/J | $3373.50 |
Frozen Mouse Embryo | B6.129(Cg)-Lepr<tm2(cre)Rck>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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