Mice that are homozygous for the mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Screening of third generation mice (G3) on atherogenic diet identified a 9 week old male (born 9/12/2004) exhibiting a hyper-response to methacholine (MCH). Absolute Penh values are as follows: 5mg/ml MCH 0.1.37(0.98), 10 mg/ml MCH 2.01(1.94), 20 mg/ml MCH 11.47(5.28). Second run values are in parenthesis. Two siblings were similarly affected. This phenotype has been validated by an invasive resistance evaluation using a flexivent protocol. This mutant mouse strain may be useful in studies of asthma and airway hyper-responsiveness (AHR).
This phenotypic deviant was identified following multidose ethylnitrosourea (ENU) treatments to induce mutations in male founder C57BL/6J mice (Stock No. 000664), in complex heart, lung, blood, and sleep disorders at the Mouse Heart, Lung, Blood, and Sleep Disorders (HLBS) Center at The Jackson Laboratory.
|Allele Name||heart, lung and blood, 454|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||hlb454, heart, lung and blood, 454|
|Strain of Origin||C57BL/6J|
|Molecular Note||This mutation was identified in an ENU mutagenesis screen for heart, lung or blood-related phenotypes.|