C3.129S7(B6)-Ifng^tm1Ts/J

Stock number: 008228
Research areas: Cancer Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

Mice that are homozygous for this Ifng (interferon-gamma) targeted mutation are resistant to experimentally induced alopecia areata. Leukocytes from homozygotes do not respond to antigens or activated inflammatory cells. This mutant mouse strain may be useful in studies of immune response, inflammation and autoimmunity.

Detailed description

Leukocytes do not respond to and Th1 cells are not activated by skin grafts in homozygous mice. Homozygotes are resistant to experimentally induced alopecia areata with diminished T cell infiltration in skin and reduced MHC class I and II molecule expression in the hair follicle infrainfundibular site. No gene product (protein) is detected by ELISA analysis of splenocyte supernatants from homozygous animals. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of immune response, inflammation and autoimmunity.

Development

A targeting vector containing a neo cassette was used to disrupt exon 2. The construct was electroporated into 129S7/SvEvBrd-Hprt1⁺ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6J blastocysts. The resulting mice were backcrossed onto the C57BL/6 background for eight generations by the donating investigator before arriving at The Jackson Laboratory. These B6.129S7-Ifng^tm1Ts/J (Stock No. 002287) mice were then backcrossed to C3H/HeJ for nine generations.

Allele

Symbol: Ifng^tm1Ts
Name: targeted mutation 1, Timothy Stewart
MGI accession ID: MGI:1857184
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ifng
Marker name: interferon gamma
Chromosome: 10
Strain of origin: 129S7/SvEvBrd-Hprt1⁺
Molecular note: Insertion of a neomycin gene into exon 2, which introduced a termination codon after the first 30 amino acids of the mature protein.