Mice that are homozygous for this Ifng (interferon-gamma) targeted mutation are resistant to experimentally induced alopecia areata. Leukocytes from homozygotes do not respond to antigens or activated inflammatory cells. This mutant mouse strain may be useful in studies of immune response, inflammation and autoimmunity.
John P Sundberg, The Jackson Laboratory
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ifng | interferon gamma |
Leukocytes do not respond to and Th1 cells are not activated by skin grafts in homozygous mice. Homozygotes are resistant to experimentally induced alopecia areata with diminished T cell infiltration in skin and reduced MHC class I and II molecule expression in the hair follicle infrainfundibular site. No gene product (protein) is detected by ELISA analysis of splenocyte supernatants from homozygous animals. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of immune response, inflammation and autoimmunity.
A targeting vector containing a neo cassette was used to disrupt exon 2. The construct was electroporated into 129S7/SvEvBrd-Hprt1+ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6J blastocysts. The resulting mice were backcrossed onto the C57BL/6 background for eight generations by the donating investigator before arriving at The Jackson Laboratory. These B6.129S7-Ifngtm1Ts/J (Stock No. 002287) mice were then backcrossed to C3H/HeJ for nine generations.
Allele Name | targeted mutation 1, Timothy Stewart |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | gko; ifgtm1; Ifntm/Ts; ifntm1Ts; IFN-g KO; ifng-; Ifngtm1Ts; Ifn-gtm1Ts; IFN-gamma-; IFNgamma-; IFNgamma KO; IFN-gamma KO; IFN-gammatm1Ts; IFN-gammaKO; Ifngtm1Ts |
Gene Symbol and Name | Ifng, interferon gamma |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 10 |
Molecular Note | Insertion of a neomycin gene into exon 2, which introduced a termination codon after the first 30 amino acids of the mature protein. |
Mutations Made By | Dr. Timothy Stewart |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the C3.129S7(B6)-Ifngtm1Ts/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008228 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Ifng<tm1Ts> |
Frozen Mouse Embryo | C3.129S7(B6)-Ifng<tm1Ts>/J | $2595.00 |
Frozen Mouse Embryo | C3.129S7(B6)-Ifng<tm1Ts>/J | $2595.00 |
Frozen Mouse Embryo | C3.129S7(B6)-Ifng<tm1Ts>/J | $3373.50 |
Frozen Mouse Embryo | C3.129S7(B6)-Ifng<tm1Ts>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.