Mice that are homozygous for this Ifng (interferon-gamma) targeted mutation are resistant to experimentally induced alopecia areata. Leukocytes from homozygotes do not respond to antigens or activated inflammatory cells. This mutant mouse strain may be useful in studies of immune response, inflammation and autoimmunity.
John P Sundberg, The Jackson Laboratory
Leukocytes do not respond to and Th1 cells are not activated by skin grafts in homozygous mice. Homozygotes are resistant to experimentally induced alopecia areata with diminished T cell infiltration in skin and reduced MHC class I and II molecule expression in the hair follicle infrainfundibular site. No gene product (protein) is detected by ELISA analysis of splenocyte supernatants from homozygous animals. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of immune response, inflammation and autoimmunity.
A targeting vector containing a neo cassette was used to disrupt exon 2. The construct was electroporated into 129S7/SvEvBrd-Hprt1+ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6J blastocysts. The resulting mice were backcrossed onto the C57BL/6 background for eight generations by the donating investigator before arriving at The Jackson Laboratory. These B6.129S7-Ifngtm1Ts/J (Stock No. 002287) mice were then backcrossed to C3H/HeJ for nine generations.
|Allele Name||targeted mutation 1, Timothy Stewart|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||gko; ifgtm1; Ifntm/Ts; ifntm1Ts; IFN-g KO; ifng-; Ifngtm1Ts; Ifn-gtm1Ts; IFN-gamma-; IFNgamma-; IFNgamma KO; IFN-gamma KO; IFN-gammatm1Ts; IFN-gammaKO; Ifngtm1Ts|
|Gene Symbol and Name||Ifng, interferon gamma|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|Molecular Note||Insertion of a neomycin gene into exon 2, which introduced a termination codon after the first 30 amino acids of the mature protein.|
|Mutations Made By|| |
Dr. Timothy Stewart
When maintaining a live colony, these mice can be bred as homozygotes.
When using the C3.129S7(B6)-Ifngtm1Ts/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008228 in your Materials and Methods section.