Mice that are homozygous for this targeted mutation exhibit reduced flash electroretinograms (rod/cone a-wave, b-wave, and cone b-wave) when compared to wildtype controls. This mutant mouse strain may be useful in studies of extracellular retinal pH and carbon dioxide regulation, photoreceptor response and retinal function.
William S Sly, Saint Louis University Medical Center
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR or Western blot analysis of brain, heart, kidney, liver, and muscle tissue. Homozygotes exhibit reduced flash electroretinograms (rod/cone a-wave, b-wave, and cone b-wave) when compared to wildtype controls. The abnormal retinal light response of homozygotes is stable over time. This mutant mouse strain may be useful in studies of extracellular retinal pH and carbon dioxide regulation, photoreceptor response and retinal function.
A tACE-CRE-neor targeting vector containing neomycin resistance, cre recombinase and the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) genes was used to disrupt exon 4 (which encodes 2 active-site His residues), 16 bp of intron 3, and 13 bp of intron 4, resulting in early termination in exon 5. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ KitlSl-J/J derived W9.5s embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The self-excising targeting vector is removed during spermatogenesis in the male chimeric animals, leaving one loxP site. The mice were then backcrossed to C57BL/6 for 20 generations before arriving at The Jackson Laboratory.
|Allele Name||targeted mutation 1, William S Sly|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CA XIV KO; CAXIVdl1Sws|
|Gene Symbol and Name||Car14, carbonic anhydrase 14|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ KitlSl-J/J|
|General Note||ES cell line = W9.5s|
|Molecular Note||A targeting vector was used to replace exon 4, 16 bp of intron 3, and 13 bp of intron 4 with the self-excising tACE-CRE-neo cassette. The frameshift resulting from recombination putatively results in early termination in exon 5. RT-PCR failed to detect the predicted 0.75 fragment in brain, heart, kidney, liver or muscle samples. Protein was not detected in Western blot analysis of the same tissues.|
|Mutations Made By|| |
William Sly, Saint Louis University Medical Center
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S1-Car14tm1Sly/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008218 in your Materials and Methods section.