B6.Cg-Tg(NPHS2-cre)295Lbh/J

Stock number: 008205
Product name: 2.5P-Cre
Research areas: Research Tools

Description

These transgenic mice (commonly referred to as podocin-Cre mice) have expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region and may be useful in generating Cre-lox conditional knockouts for studying the role of podocyte nephrobiology in renal disorders.

Detailed description

Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) are viable and fertile, with expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region. Cre-recombinase activity is reported in podocytes during late capillary loop stage of glomerular development and persists in podocytes of mature glomeruli, with no evidence for cre expression detected in other tissues examined. Embryonic Cre-recombinase activity is also reported as early as 8.5 dpc. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the sequence. These Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) may be useful in generating conditional knockouts for studying the role of podocyte nephrobiology in renal disorders.

Development

The p2.5P-Cre transgene was designed with a 2.5 kb fragment of the human podocin (or NPHS2) promoter/enhancer region upstream of a Cre recombinase cassette followed by a murine protamine poly-adenylation signal. The donating investigator reports that the transgene was microinjected into (C57BL/6 X SJL)F2 mouse eggs. Mice from founder line 295 were found to have podocyte-specific cre activity, were subsequently backcrossed to C57BL/6J for at least ten generations, and then bred together to generate "homozygotes" prior to arrival at The Jackson Laboratory Repository. Upon arrival, the 2.5P-Cre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

In 2017, a 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 4, was segregating with SJL.

Allele

Symbol: Tg(NPHS2-cre)295Lbh
Name: transgene insertion 295, Lawrence B Holzman
MGI accession ID: MGI:2450359
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(NPHS2-cre)295Lbh
Marker name: transgene insertion 295, Lawrence B Holzman
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase expression in podocytes within kidney glomeruli.
Molecular note: The human NPHS2 promoter, including the entire 5' untranslated region, was used to drive cre recombinase expression in podocytes.
General note: 24 transgenic founders were created. Phenotypic analysis was performed on founder line #295.