These transgenic mice (commonly referred to as podocin-Cre mice) have expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region and may be useful in generating Cre-lox conditional knockouts for studying the role of podocyte nephrobiology in renal disorders.
Lawrence B Holzman, University of Pennsylvania
Genetic Background | Generation |
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N11+N3F13
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$328.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) are viable and fertile, with expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region. Cre-recombinase activity is reported in podocytes during late capillary loop stage of glomerular development and persists in podocytes of mature glomeruli, with no evidence for cre expression detected in other tissues examined. Embryonic Cre-recombinase activity is also reported as early as 8.5 dpc. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the sequence. These Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) may be useful in generating conditional knockouts for studying the role of podocyte nephrobiology in renal disorders.
The p2.5P-Cre transgene was designed with a 2.5 kb fragment of the human podocin (or NPHS2) promoter/enhancer region upstream of a Cre recombinase cassette followed by a murine protamine poly-adenylation signal. The donating investigator reports that the transgene was microinjected into (C57BL/6 X SJL)F2 mouse eggs. Mice from founder line 295 were found to have podocyte-specific cre activity, were subsequently backcrossed to C57BL/6J for at least ten generations, and then bred together to generate "homozygotes" prior to arrival at The Jackson Laboratory Repository. Upon arrival, the 2.5P-Cre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | cre recombinase expression in podocytes within kidney glomeruli. |
Allele Name | transgene insertion 295, Lawrence B Holzman |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | 2.5P-Cre; NPHS2.Cre; pod-Cre; podocin-Cre; PodoCre; Tg(NPHS2-cre)1Lbh |
Gene Symbol and Name | Tg(NPHS2-cre)295Lbh, transgene insertion 295, Lawrence B Holzman |
Gene Synonym(s) | |
Promoter | NPHS2, NPHS2, podocin, human |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | cre recombinase expression in podocytes within kidney glomeruli. |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
General Note | 24 transgenic founders were created. Phenotypic analysis was performed on founder line #295. |
Molecular Note | The human NPHS2 promoter, including the entire 5' untranslated region, was used to drive cre recombinase expression in podocytes. |
Mutations Made By | Lawrence Holzman, University of Pennsylvania |
When maintaining a live colony, hemizygous mice may be bred with wildtype siblings or with C57BL/6J inbred mice. The donating investigator reports that homozygous mice may be bred together.
When using the 2.5P-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #008205 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(NPHS2-cre)295Lbh |
Frozen Mouse Embryo | B6.Cg-Tg(NPHS2-cre)295Lbh/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(NPHS2-cre)295Lbh/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(NPHS2-cre)295Lbh/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(NPHS2-cre)295Lbh/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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