Overview

These Gata6^loxp mice may be useful in generating conditional GATA binding protein 6 mutations for studying GATA6 function in organogenesis or in adult mice.

Donating Investigator

Stephen Duncan, Medical College of Wisconsin

Genetic overview

Genetic Background	Generation

Gata6^tm2.1Sad

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Gata6	GATA binding protein 6

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Research Applications

Research Tools

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for this Gata6^loxp conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the majority of the GATA6 protein) deleted in the cre-expressing tissue(s). These Gata6^loxp mice may be useful in generating conditional GATA binding protein 6 mutations for studying GATA6 function in organogenesis or in adult mice.

For example, when bred to a strain expressing Cre recombinase in the villi and crypt cells of the intestine (see Stock No. 004586 for example), or when bred to a strain expressing Cre recombinase in the embryo (see Stock No. 003724, 003314 for example), this mutant mouse strain may be useful in studies of the transcription factor Gata6.

When bred to
B6.Cg-Tg(Prrx1-cre)1Cjt/J mice (Stock No. 005584) expressing Cre recombinase in limb bud mesenchyme, offspring exhibit preaxial hindlimb polydactyly.

Development

A targeting vector was designed to place a loxP site and frt-flanked neo cassette upstream of exon 2, and a second loxP site just downstream of exon 2 of the targeted gene. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Chimeric mice were bred with CD-1 mice. To remove the selection cassette, these Gata6^{loxp(FRTneoFRT)} mice were bred to FLPe-expressing mice (on a B6;SJL genetic background, see Stock No. 003800). The resulting Gata6^loxp offspring were then bred to CD-1 mice to remove the transgene. These Gata6^loxp mice were maintained on a mixed genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to 129S1/SvImJ for at least one generation to establish the colony.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Sodhi CP; Li J; Duncan SA. 2006. Generation of mice harbouring a conditional loss-of-function allele of Gata6. BMC Dev Biol 6:19PubMed: 16611361 MGI: J:111388

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Genetics

Gata6^tm2.1Sad

Allele Symbol: Gata6^tm2.1Sad

Allele Name	targeted mutation 2.1, Stephen A Duncan
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Gata6 floxed; Gata6^loxP
Gene Symbol and Name	Gata6, GATA binding protein 6
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	18
General Note	This allele was derived by deletion of an FRT-flanked neomycin phosphotransferase gene that in the original construct lay just downstream of the 5' loxP site. Mice homozygous for the neo-containing allele could not be recovered from heterozygous matings. (J:111388)
Molecular Note	A pair of loxP sequences are located in the first and second introns, flanking exon 2. A single FRT sequence is present immediately downstream of the more 5' loxP as a consequence of the removal of an frt-flanked neomycin seletion cassette.
Mutations Made By	Stephen Duncan, Medical College of Wisconsin

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - loxP-flanked Sequences
- Developmental Biology Research
  - Cre-lox System
- Cardiovascular Research
  - Cre-lox System

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Gata6^tm2.1Sad/Gata6^tm2.1Sad Tg(Prrx1-cre)1Cjt/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * SJL/J

limbs/digits/tail phenotype

preaxial polydactyly
- 100% penetrance of preaxial hindlimb polydactyly with a variable number of supernumerary digits
- forelimb digits are not affected

Genotype: Gata6^tm2.1Sad/Gata6^tm2.2Sad Tg(EIIa-cre)C5379Lmgd/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * FVB/N * SJL

mortality/aging

embryonic lethality during organogenesis, complete penetrance
- between E8.5 and E11.5 all Cre positive double mutants appear to be partially resorbed empty decidual masses

Genotype: Gata6^tm2.1Sad/Gata6^tm2.1Sad Tg(Vil1-cre)997Gum/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL

mortality/aging

embryonic lethality during organogenesis, complete penetrance
- matings of 4 of 5 males carrying the Cre transgene to homozygous females produced no offspring homozygous for the floxed allele and Cre positive

The following phenotype relates to a compound genotype created using this strain

Genotype: Gata6^tm2.1Sad/Gata6^tm2.1Sad
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL

normal phenotype

no abnormal phenotype detected
- Normal - viable and fertile with no gross malformations

References

Sodhi CP; Li J; Duncan SA. 2006. Generation of mice harbouring a conditional loss-of-function allele of Gata6. BMC Dev Biol 6:19PubMed: 16611361 MGI: J:111388

Additional - Gata6^tm2.1Sad related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Gata6
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Gata6^tm2.1Sad/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008196 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Heterozygous for Gata6<tm2.1Sad>

Related Products and Services

Frozen Mouse Embryo	STOCK Gata6<tm2.1Sad>/J	$2595.00
Frozen Mouse Embryo	STOCK Gata6<tm2.1Sad>/J	$2595.00
Frozen Mouse Embryo	STOCK Gata6<tm2.1Sad>/J	$3373.50
Frozen Mouse Embryo	STOCK Gata6<tm2.1Sad>/J	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Gata6tm2.1Sad

Allele Symbol: Gata6tm2.1Sad

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gata6tm2.1Sad/Gata6tm2.1Sad Tg(Prrx1-cre)1Cjt/0involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * SJL/J

limbs/digits/tail phenotype

Genotype: Gata6tm2.1Sad/Gata6tm2.2Sad Tg(EIIa-cre)C5379Lmgd/0involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * FVB/N * SJL

mortality/aging

Genotype: Gata6tm2.1Sad/Gata6tm2.1Sad Tg(Vil1-cre)997Gum/0involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL

mortality/aging

Genotype: Gata6tm2.1Sad/Gata6tm2.1Sadinvolves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL

normal phenotype

References

Additional - Gata6tm2.1Sad related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Gata6^tm2.1Sad

Allele Symbol: Gata6^tm2.1Sad

Genotype: Gata6^tm2.1Sad/Gata6^tm2.1Sad Tg(Prrx1-cre)1Cjt/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * SJL/J

Genotype: Gata6^tm2.1Sad/Gata6^tm2.2Sad Tg(EIIa-cre)C5379Lmgd/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * FVB/N * SJL

Genotype: Gata6^tm2.1Sad/Gata6^tm2.1Sad Tg(Vil1-cre)997Gum/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL

Genotype: Gata6^tm2.1Sad/Gata6^tm2.1Sad
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL

Additional - Gata6^tm2.1Sad related