STOCK Gata6^tm2.1Sad/J

Stock number: 008196
Research areas: Research Tools

Description

These Gata6^loxp mice may be useful in generating conditional GATA binding protein 6 mutations for studying GATA6 function in organogenesis or in adult mice.

Detailed description

Mice homozygous for this Gata6^loxp conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the majority of the GATA6 protein) deleted in the cre-expressing tissue(s). These Gata6^loxp mice may be useful in generating conditional GATA binding protein 6 mutations for studying GATA6 function in organogenesis or in adult mice.

For example, when bred to a strain expressing Cre recombinase in the villi and crypt cells of the intestine (see Stock No. 004586 for example), or when bred to a strain expressing Cre recombinase in the embryo (see Stock No. 003724, 003314 for example), this mutant mouse strain may be useful in studies of the transcription factor Gata6.

When bred to B6.Cg-Tg(Prrx1-cre)1Cjt/J mice (Stock No. 005584) expressing Cre recombinase in limb bud mesenchyme, offspring exhibit preaxial hindlimb polydactyly.

Development

A targeting vector was designed to place a loxP site and frt-flanked neo cassette upstream of exon 2, and a second loxP site just downstream of exon 2 of the targeted gene. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Chimeric mice were bred with CD-1 mice. To remove the selection cassette, these Gata6^{loxp(FRTneoFRT)} mice were bred to FLPe-expressing mice (on a B6;SJL genetic background, see Stock No. 003800). The resulting Gata6^loxp offspring were then bred to CD-1 mice to remove the transgene. These Gata6^loxp mice were maintained on a mixed genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to 129S1/SvImJ for at least one generation to establish the colony.

Allele

Symbol: Gata6^tm2.1Sad
Name: targeted mutation 2.1, Stephen A Duncan
MGI accession ID: MGI:3654150
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Gata6
Marker name: GATA binding protein 6
Chromosome: 18
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A pair of loxP sequences are located in the first and second introns, flanking exon 2. A single FRT sequence is present immediately downstream of the more 5' loxP as a consequence of the removal of an frt-flanked neomycin seletion cassette.
General note: This allele was derived by deletion of an FRT-flanked neomycin phosphotransferase gene that in the original construct lay just downstream of the 5' loxP site. Mice homozygous for the neo-containing allele could not be recovered from heterozygous matings. (J:111388)