These Gata6loxp mice may be useful in generating conditional GATA binding protein 6 mutations for studying GATA6 function in organogenesis or in adult mice.
Stephen Duncan, Medical College of Wisconsin
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Gata6 | GATA binding protein 6 |
Mice homozygous for this Gata6loxp conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the majority of the GATA6 protein) deleted in the cre-expressing tissue(s). These Gata6loxp mice may be useful in generating conditional GATA binding protein 6 mutations for studying GATA6 function in organogenesis or in adult mice.
For example, when bred to a strain expressing Cre recombinase in the villi and crypt cells of the intestine (see Stock No. 004586 for example), or when bred to a strain expressing Cre recombinase in the embryo (see Stock No. 003724, 003314 for example), this mutant mouse strain may be useful in studies of the transcription factor Gata6.
When bred to
B6.Cg-Tg(Prrx1-cre)1Cjt/J mice (Stock No. 005584) expressing Cre recombinase in limb bud mesenchyme, offspring exhibit preaxial hindlimb polydactyly.
A targeting vector was designed to place a loxP site and frt-flanked neo cassette upstream of exon 2, and a second loxP site just downstream of exon 2 of the targeted gene. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Chimeric mice were bred with CD-1 mice. To remove the selection cassette, these Gata6loxp(FRTneoFRT) mice were bred to FLPe-expressing mice (on a B6;SJL genetic background, see Stock No. 003800). The resulting Gata6loxp offspring were then bred to CD-1 mice to remove the transgene. These Gata6loxp mice were maintained on a mixed genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to 129S1/SvImJ for at least one generation to establish the colony.
Allele Name | targeted mutation 2.1, Stephen A Duncan |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Gata6 floxed; Gata6loxP |
Gene Symbol and Name | Gata6, GATA binding protein 6 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 18 |
General Note | This allele was derived by deletion of an FRT-flanked neomycin phosphotransferase gene that in the original construct lay just downstream of the 5' loxP site. Mice homozygous for the neo-containing allele could not be recovered from heterozygous matings. (J:111388) |
Molecular Note | A pair of loxP sequences are located in the first and second introns, flanking exon 2. A single FRT sequence is present immediately downstream of the more 5' loxP as a consequence of the removal of an frt-flanked neomycin seletion cassette. |
Mutations Made By | Stephen Duncan, Medical College of Wisconsin |
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Gata6tm2.1Sad/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008196 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gata6<tm2.1Sad> |
Frozen Mouse Embryo | STOCK Gata6<tm2.1Sad>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gata6<tm2.1Sad>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Gata6<tm2.1Sad>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Gata6<tm2.1Sad>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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