These mice possess loxP sites on either side of exon 3 of the Rb1 (retinoblastoma 1) gene. This strain may be useful in cancer or cell cycle/senescence studies.
Dr. Tyler Jacks, Massachusetts Institute of Technology
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this conditional mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This strain may be useful in cancer or cell cycle/senescence studies.
A targeting vector was designed to introduce a puromycin resistance cassette flanked by loxP sites to intron 3, and an additional loxP site to intron 2. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Transient Cre excision was used to excise the puromycin cassette, leaving exon 3 flanked by loxP sites. This strain was maintained on a mixed background composed of C57BL/6 and 129 by the donating laboratory.
|Allele Name||targeted mutation 3, Tyler Jacks|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||targeted mutation 3, Tyler Jacks; Rb1tm3Tyj|
|Gene Symbol and Name||Rb1, RB transcriptional corepressor 1|
|Gene Synonym(s)||retinoblastoma 1; Rb-1; pp110; RB; Rb-1; pRb; OSRC; p105-Rb; PPP1R130; Rb; retinoblastoma 1; p110-RB1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Single loxP sites flank exon 3.|
|Mutations Made By|| |
Dr. Tyler Jacks, Massachusetts Institute of Technology
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129-Rb1tm3Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008186 in your Materials and Methods section.
|Heterozygous or wild-type for Rb1<tm3Tyj>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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